Cm1860 uv

Manufactured by Leica Microsystems

Sourced in Germany

The Leica CM1860 UV is a cryostat for histological sectioning. It is designed to enable precise and consistent cutting of frozen tissue samples. The instrument features a stable microtome for controlled sectioning and a UV-C disinfection system to maintain a sterile work environment.

Automatically generated - may contain errors

Lab products found in correlation

Superfrost plus slide, by Thermo Fisher Scientific (2 mentions) Normal rabbit serum, by Thermo Fisher Scientific (1 mentions) Camedia c 2000 digital camera, by Olympus (1 mentions) Anti hmgb1 rabbit polyclonal antibodies, by Novus Biologicals (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Bx40 microscope, by Olympus (1 mentions)

3 protocols using cm1860 uv

Immunohistochemical Analysis of HMGB1

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Amniotic membranes were embedded in Tissue-Tek (Sakura, Tokyo, Japan), snap-frozen in isopentane cooled in liquid nitrogen vapor and stored at −70 °C. Five μm acetone-fixed cryosections were cut on a cryostat CM1860 UV (Leica Microsystems, Wetzlar, Germany), put on SuperFrost/Plus slides (Thermo Fisher Scientific, Darmstadt, Germany), and were kept at −40 °C until labeling. Later, the sections were processed as described elsewhere [33 (link)]. Briefly, they were blocked with 10% normal rabbit serum (Life Technologies, Carlsbad, CA, USA) for 1 h at RT, incubated with anti-HMGB1 rabbit polyclonal antibodies (Novus Biologicals, Centennial, CO, USA) for 16 h at 4 °C, and labeled with Alexa Fluor 488-conjugated goat anti-rabbit IgG antibodies (Life Technologies) for 2 h at RT After embedding in ProLong Gold Antifade Reagent (Life Technologies), the sections were evaluated under an Olympus BX 40 microscope with an Olympus Camedia C-2000 digital camera (Olympus, Tokyo, Japan). The sections without primary antibodies served as controls. The HMGB1 and nuclei colocalization was verified by ImageJ software [34 (link)].

Splichal I, & Splichalova A. (2021). High Mobility Group Box 1 in Pig Amniotic Membrane Experimentally Infected with E. coli O55. Biomolecules, 11(8), 1146.

+ Open protocol

+ Expand

Brain Tissue Harvesting and Cryoprotection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The brain collection was performed 21 days after stereotaxic injection. Mice were anaesthetized by isoflurane and were perfused with ice-cold PBS followed by 4% PFA in PBS. The brains were postfixed for 4 hr in 4% PFA, washed once with PBS, and then cryoprotected by 30% sucrose in PBS at 4 °C for 2–3 days. After cryoprotection, the brains were embedded in O.C.T. compound, frozen, and then stored at −80 °C until further processing. The brain sections were obtained by cryostat (CM1860 UV; Leica Microsystems). Slices with striatum and hippocampus were sectioned on the coronal plane at 20 μm, mounted on the glass slides, and stored at −20 °C until further use. The sections for DAPI staining without further immunostaining were washed three times in PBS. After the washing, the sections were incubated with DAPI diluted in PBS (1:1500 of 5 μg μL⁻¹ stock solution) for 10 min. Next, the sections were washed three times in PBS and mounted with Prolong Gold Antifade Reagent for imaging.

Tuma J., Chen Y.J., Collins M.G., Paul A., Li J., Han H., Sharma R., Murthy N, & Lee H.Y. (2023). Lipid nanoparticles deliver mRNA to the brain after an intracerebral injection. Biochemistry, 62(24), 3533-3547.

+ Open protocol

+ Expand

Cryosectioning Lung Tissue for Budesonide and SP-C Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cryostat CM1860UV (Leica Microsystems, Wetzlar, Germany) was used for sectioning the lung lobes as fresh frozen tissue. The lobes were cut among transversal plane at middle height obtaining tissue section of approximately 50 × 20 mm. Two consecutive sections were cut at 20 µm thickness: one section was thaw-mounted over a Superfrost slide for budesonide analysis and the second one was thaw-mounted over an ITO slide for SP-C analysis (Figure 1). All the prepared glass slides were posed in a vacuum desiccator (15 min) and allowed to dry prior further phases of sample preparation.

Zecchi R., Franceschi P., Tigli L., Pioselli B., Mileo V., Murgia X., Salomone F., Pieraccini G., Usada H., Schmidt A.F., Hillman N.H., Kemp M.W, & Jobe A.H. (2021). Surfactant-Assisted Distal Pulmonary Distribution of Budesonide Revealed by Mass Spectrometry Imaging. Pharmaceutics, 13(6), 868.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!