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Cd45 h130

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CD45 (H130) is a laboratory reagent used for the identification and enumeration of leukocytes. It is a monoclonal antibody that specifically binds to the CD45 antigen expressed on the surface of various types of white blood cells, including T cells, B cells, and monocytes. The primary function of CD45 (H130) is to serve as a marker for the detection and quantification of these cell populations in research and clinical settings.

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Lab products found in correlation

Pe cd34 pool, by Beckman Coulter (2 mentions) Luc90, by Creative Biolabs (2 mentions) Yeti flow cytometer, by Tree Star (2 mentions) Cd19 sj25c1, by BD (1 mentions) Cd123 fab301c, by R&D Systems (1 mentions) Cd11c bu15, by Abcam (1 mentions) Crth2 bm16, by Miltenyi Biotec (1 mentions) Cd3 sk7, by BD (1 mentions) Cd11b dcis1 18, by Abcam (1 mentions) Fcεri aer 37 cra 1, by BD (1 mentions) Live dead violet, by Thermo Fisher Scientific (1 mentions) Cd45 hi30, by BD (1 mentions) Il 7rα a019d5, by Thermo Fisher Scientific (1 mentions)

3 protocols using cd45 h130

1

FACS Surface Staining of Immune Cells

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For FACS surface staining, the cells were labelled by the following anti-human antibodies: CD3 (SK7; BD), CD19 (SJ25C1; BD), CD123 (FAB301C; R&D Systems), CD11b (DCIS1/18), CD11c (BU15; Abcam), CD4 (A161A1, BD), CD8 (RPA-T8), FcεRI (AER-37 ([CRA-1]), CD14 (MϕP9; BD), CD45 (H130), CD56 (B159), CD45 (HI30, BD), CRTH2 (BM16; Miltenyi Biotec), IL-7Rα (A019D5), and live/dead violet (Invitrogen). The samples were acquired using FACSDiva on LSRFortessa flow cytometer. FlowJo software (FlowJo_v10.7.1) was used for data analysis.
Fonseka C.L., Hardman C.S., Woo J., Singh R., Nahler J., Yang J., Chen Y.L., Kamaladasa A., Silva T., Salimi M., Gray N., Dong T., Malavige G.N, & Ogg G.S. (2022). Dengue virus co-opts innate type 2 pathways to escape early control of viral replication. Communications Biology, 5, 735.
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2

Multiparameter Flow Cytometry Analysis

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Antibodies used were CS1 (162.1), CD14 (M5E2), CD20 (2H7), CD19 (HIB19), IgG secondary, CD8 (RPA-T8), CD38 (BioLegend); CD4 (RPA-T4 or SK3) CD8 (RPA-T8), CD3 (UCHT1), CD56 (NCAM16.2), CD33 (WM53), CD45 (H130) BD Pharmingen; PE-CD34 Pool (Beckman Coulter), CD138, BCMA (Miltenyi), purified Luc90 (Creative Biolabs). 7AAD or Dapi was used for viability. Samples were run on an Attune or Yeti Flow Cytometer and analyzed using FlowJo V10 (TreeStar, Ashland, OR).
O’Neal J., Ritchey J.K., Cooper M.L., Niswonger J., Sofía González L., Street E., Rettig M.P., Gladney S.W., Gehrs L., Abboud R., Prior J.L., Haas G.J., Jayasinghe R.G., Ding L., Ghobadi A., Vij R, & DiPersio J.F. (2022). CS1 CAR-T targeting the distal domain of CS1 (SLAMF7) shows efficacy in high tumor burden myeloma model despite fratricide of CD8+CS1 expressing CAR-T cells. Leukemia, 36(6), 1625-1634.
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3

Multiparameter Flow Cytometry Analysis

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Antibodies used were CS1 (162.1), CD14 (M5E2), CD20 (2H7), CD19 (HIB19), IgG secondary, CD8 (RPA-T8), CD38 (BioLegend); CD4 (RPA-T4 or SK3) CD8 (RPA-T8), CD3 (UCHT1), CD56 (NCAM16.2), CD33 (WM53), CD45 (H130) BD Pharmingen; PE-CD34 Pool (Beckman Coulter), CD138, BCMA (Miltenyi), purified Luc90 (Creative Biolabs). 7AAD or Dapi was used for viability. Samples were run on an Attune or Yeti Flow Cytometer and analyzed using FlowJo V10 (TreeStar, Ashland, OR).
O’Neal J., Ritchey J.K., Cooper M.L., Niswonger J., Sofía González L., Street E., Rettig M.P., Gladney S.W., Gehrs L., Abboud R., Prior J.L., Haas G.J., Jayasinghe R.G., Ding L., Ghobadi A., Vij R, & DiPersio J.F. (2022). CS1 CAR-T targeting the distal domain of CS1 (SLAMF7) shows efficacy in high tumor burden myeloma model despite fratricide of CD8+CS1 expressing CAR-T cells. Leukemia, 36(6), 1625-1634.
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