Pclxsn gfp

Manufactured by Takara Bio

The PCLXSN-GFP is a viral vector product from Takara Bio. It is a replication-incompetent lentiviral vector that expresses the green fluorescent protein (GFP) transgene.

Automatically generated - may contain errors

Lab products found in correlation

Pegfp n3, by Takara Bio (2 mentions) Pcmv5 myc, by Qiagen (2 mentions) Hiload 16 60 superdex 200 column, by Cytiva (2 mentions) Pgex 5x 3, by Cytiva (2 mentions) Pcdna3.1 v5 his, by Thermo Fisher Scientific (2 mentions) Glutathione sepharose beads, by Cytiva (2 mentions)

2 protocols using pclxsn gfp

Cloning and Purification of rMap7D2 Protein

Cited 1 time

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Based on the information of DDBJ/EMBL/GenBank accession number NM_001289778.1, oligonucleotide primers (5′-ATGTCGACATGGAGCGCAGCGGTGGGAACGGCG-3′ and 5′-ATGTCGACTCAACAGAAGGTGTTCAGCGTAGTTTC-3′) were designed, and rat Map7d2 (rMap7d2) cDNA was obtained by PCR using rat cDNA as a template. Expression vectors for rMap7d2 were constructed in pCMV5-Myc (Nakanishi et al, 1997 (link)), pQE9 (QIAGEN), pGEX-5X-3 (Cytiva), pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al, 2005 (link)), and pEGFP-N3 (Clontech). GST-fused proteins were expressed in Escherichia coli and purified using glutathione-Sepharose beads (Cytiva), respectively. GST-rMap7D2 (full length) was further purified by gel filtration using a HiLoad 16/60 Superdex 200 column (Cytiva).

Kikuchi K., Sakamoto Y., Uezu A., Yamamoto H., Ishiguro K.I., Shimamura K., Saito T., Hisanaga S.I, & Nakanishi H. (2022). Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells. Life Science Alliance, 5(8), e202201390.

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Cloning and Expression of rat Map7d2

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Based on the information of DDBJ/EMBL/GenBank accession number XM_228973, oligonucleotide primers (5′-ATGTCGACATGGAGCGCAGCGGTGGGAACGGCG-3′ and 5-ATGTCGACTCAACAGAAGGTGTTCAGCGTAGTTTC-3′) were designed, and rat Map7d2 (rMap7d2) cDNA was obtained by PCR using rat cDNA as a template. Expression vectors for rMap7d2 were constructed in pCMV5-Myc (Nakanishi 1997) , pQE9 (Qiagen), pGEX-5X-3 (Cytiva), pcDNA3.1/V5-His (Thermo Fisher Scientific), pCLXSN-GFP (Reiley et al 2005) , and pEGFP-N3 (Clontech). GST-fused proteins were expressed in Escherichia coli and purified using glutathione-Sepharose beads (Cytiva), respectively. GST-rMap7D2 (full length) was further purified by gel filtration using a HiLoad 16/60 Superdex 200 column (Cytiva).

Kikuchi K., Sakamoto Y., Uezu A., Yamamoto H., Ishiguro K., Shimamura K., Saito T., Hisanaga S., & Nakanishi H. (2021). Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cells.

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