Total RNA was extracted from the liver tissue using a High Pure RNA Isolation Kit (Roche, Mannheim, Germany). Aliquots of total RNA (2 µg) were transformed into cDNA using a cDNA reverse transcription kit (Applied Biosystems, Foster City, CA, USA). The cDNA subsequently underwent amplification for quantitative qPCR using Luna Universal Probe qPCR Master Mix (New England Biolabs, Beverly, MA, USA) and target-specific probe primer (Applied Biosystems, Foster City, CA, USA) for COL1A1, TGF-β, and IL-10. Relative expression was calculated using comparative ∆∆Ct values.
Target specific probe primer
Manufactured by Thermo Fisher Scientific
Sourced in United States
Target-specific probe primer is a laboratory equipment designed for the detection and amplification of specific DNA or RNA sequences. It consists of a short, synthetic oligonucleotide sequence that is complementary to a target genetic region. This primer serves as a starting point for DNA or RNA replication, enabling the selective amplification of the target sequence during molecular biology techniques, such as polymerase chain reaction (PCR).
Automatically generated - may contain errors
2 protocols using target specific probe primer
1
Analyzing Gene Expression in Liver Tissue
2
RNA Isolation and qPCR from Liver Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Liver tissue samples were homogenized in TRIzol reagent (Invitrogen, Gaithersburg, MD, USA). High Pure RNA Isolation Kit (Roche) was used to isolate RNA from liver tissue. Total RNA isolation from tissue was used a cDNA reverse transcription kit (applied Biosystems, Foster City, CA, USA), and aliquots of total RNA (2 μg) were transformed into cDNA. The cDNA subsequently underwent amplification for quantitative PCR using the Luna® Universal Probe qPCR Master Mix (New England Biolabs, Beverly, MA, USA) and target-specific probe-primer (applied Biosystems).
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