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Mouse ifn alpha platinum elisa kit

Manufactured by Thermo Fisher Scientific
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The Mouse IFN alpha Platinum ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) used for the measurement of mouse interferon alpha (IFN-α) levels in biological samples. The kit utilizes pre-coated microplates and provides all the necessary reagents for performing the assay.

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Lab products found in correlation

Mouse baff blys tnfsf13b quantikine elisa kit, by R&D Systems (1 mentions)

2 protocols using mouse ifn alpha platinum elisa kit

1

Plamacytoid Dendritic Cell Stimulation

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2 × 104 mature pDCs and 2 × 103 progenitor cells were sort-purified and stimulated with CpG-A 2216 (6 μ g/ml) or CpG-B 1826 (6 μ g/ml) for 16 h at day 0 or after 4 d of culture. Supernatants were analyzed with a Mouse IFN alpha Platinum ELISA Kit (eBioscience).
Rodrigues P.F., Alberti-Servera L., Eremin A., Grajales-Reyes G.E., Ivanek R, & Tussiwand R. (2018). Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells. Nature immunology, 19(7), 711-722.
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2

Cytokine Profiling of Immune Responses

Cited 1 time
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The levels of BAFF in media, imiquimod, or ODN 2395 stimulated cell culture supernatants were analyzed using the Mouse BAFF/BLyS/TNFSF13B Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA). The levels of IFNα in serum and splenic leukocyte culture supernatants were determined with eBioscience Mouse IFN alpha Platinum ELISA kit (eBioscience). The ELISAs were performed per the manufacturers’ protocols. Ciraplex® Chemiluminescent Assay kits (Aushon Biosystem, Billerica, MA, USA) were used to quantify the levels of IFN-γ, IL-1β, IL-2, IL-6, IL-10, and TNFα in serum and cell culture supernatants per the manufacturer’s instructions49 (link),65 (link). The images of chemiluminescent array plates were captured with Cirascan image system (Aushon) and the image data was processed with Cirasoft software. All the assays were performed with two technical replicates for each sample.
Edwards M.R., Dai R., Heid B., Cowan C., Werre S.R., Cecere T, & Ahmed S.A. (2020). Low-dose 17α-ethinyl estradiol (EE) exposure exacerbates lupus renal disease and modulates immune responses to TLR7/9 agonists in genetically autoimmune-prone mice. Scientific Reports, 10, 5210.
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