Clp290

On the day of the experiment, midazolam (Sandoz, Tokyo, Japan) was diluted with 5× saline (final 1 mg/ml) to be administered at 0.02 ml/g of body weight. CLP290 was received from Dr. Yves De Koninck (Institut Universitaire en Santé Mentale de Québec) or purchased (#SML-2172, Sigma-Aldrich, St. Louis, MO, USA). CLP290 was adjusted to a final concentration of 0.66% prior to oral administration; the CLP290 from Dr. De Koninck was dissolved in 10% hydroxypropyl-β-cyclodextrin, whereas the CLP290 from Sigma-Aldrich was suspended in 0.5% carboxy methylcellulose. On the day of the experiment, VU0463271 (#4719/10, Tocris Bioscience, Bristol, UK) was dissolved in 0.75% dimethyl sulfoxide, at a final concentration of 1 mM.