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Dioleoylphosphatidylethanolamine

Manufactured by Lipoid
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Sourced in Germany, United States

Dioleoylphosphatidylethanolamine is a synthetic lipid compound commonly used in laboratory research. It is a phospholipid consisting of two oleic acid chains attached to a phosphatidylethanolamine backbone. This compound is often employed as a model system to study membrane properties and interactions in biochemical and biophysical research.

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Lab products found in correlation

Cholesteryl hemisuccinate chems, by Merck Group (3 mentions) Cholesterol chol, by Merck Group (2 mentions) Dspe peg2000 dspe peg fol, by Lipoid (2 mentions) Distearoyl phosphatidyl ethanolamine, by Lipoid (1 mentions) D glucose, by Vetec (1 mentions) Syto9, by Thermo Fisher Scientific (1 mentions) Mueller hinton broth (mhb), by Thermo Fisher Scientific (1 mentions) Mueller hinton agar (mha), by Thermo Fisher Scientific (1 mentions) Vancomycin, by Merck Group (1 mentions) Levofloxacin, by Merck Group (1 mentions) Stearylamine, by Merck Group (1 mentions) Dimyristoyl phosphatidyl choline, by Lipoid (1 mentions) Dimyristoyl phosphatidyl glycerol, by Lipoid (1 mentions) Dipalmitoyl phosphatidyl glycerol, by Lipoid (1 mentions) Tryptic soy broth tsb, by Solabia (1 mentions) Dipalmitoylphosphatidylcholine, by Lipoid (1 mentions) Cholesteryl hemisuccinate, by Avanti Polar Lipids (1 mentions) Whatman, by Cytiva (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions)

5 protocols using dioleoylphosphatidylethanolamine

1

Optimizing Irinotecan Delivery in Mice

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Trihydrated irinotecan hydrochloride (IRN) was donated from EUROFARMA Laboratórios SA (São Paulo, Brazil). The dioleoylphosphatidylethanolamine (DOPE), and distearoylphosphatidylethanolamine polyethyleneglycol2000 (DSPE-PEG2000/DSPE-PEG-FOL) were supplied by Lipoid GmbH (USA). Cholesteryl hemisuccinate (CHEMS) and cholesterol (CHOL) were purchased from Sigma-Aldrich Chemical Company (St Louis, USA). Glucose was purchased from Vetec Química Fina Ltda (São Paulo, Brazil). All solvents (high-performance liquid chromatography analytical grade) and other reagents were purchased from Sigma-Aldrich (São Paulo, Brazil). The subcutaneous tumor model was established in 8–10- week-old female BALB/c mice purchased from CEBIO-UFMG (Belo Horizonte, Brazil). Animals were kept under SPF condition with free access to standard food and water. All animal studies were approved by the local Ethics Committee for Animal Experiments of Federal University of Minas Gerais, Brazil (protocol # 102/2020).
Nunes S.S., Miranda S.E., de Oliveira Silva J., Fernandes R.S., de Alcântara Lemos J., de Aguiar Ferreira C., Townsend D.M., Cassali G.D., Oliveira M.C, & de Barros A.L. (2021). pH-responsive and folate-coated liposomes encapsulating irinotecan as an alternative to improve efficacy of colorectal cancer treatment. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 144, 112317.
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2

Formulation and Characterization of Doxorubicin-Loaded Lipid Nanoparticles

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The reagents and chemicals used in this study were of analytical grade and are commercially available. Specifically, Dioleoylphosphatidylethanolamine (DOPE, LIPOID PE 18:1/18:1), distearoylphosphatidylethanolamine (DSPE, LIPOID PE 18:0/18:0), and distearoylphosphatidylethanolamine polyethyleneglycol2000(DSPE-PEG2000, LIPOID PE 18:0/18:0–PEG2000) were supplied by Lipoid GmbH (Ludwigshafen, Germany). Cholesteryl hemisuccinate (CHEMS, Cat. # 850524P) was purchased from Sigma-Aldrich Chemical Company (St Louis, MO, USA). Doxorubicin hydrochloride (DOX, >98% purity) was purchased from ACIC Chemicals (Brantford, Ontario, Canada). D-(+)-Glucose (99,5% purity) was purchased from Vetec Química Fina Ltd.a (São Paulo, Brazil). The analytical reagents and solvents (high-performance liquid chromatography analytical grade) were purchased from Sigma-Aldrich (São Paulo, Brazil).
Nunes S.S., de Oliveira Silva J., Fernandes R.S., Miranda S.E., Leite E.A., de Farias M.A., Portugal R.V., Cassali G.D., Townsend D.M., Oliveira M.C, & de Barros A.L. (2022). PEGylated versus Non-PEGylated pH-Sensitive Liposomes: New Insights from a Comparative Antitumor Activity Study. Pharmaceutics, 14(2), 272.
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3

Nano-formulations for Antimicrobial Delivery

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Levofloxacin (LEV) and vancomycin (VCM) were obtained from Sigma–Aldrich (St. Louis, MO, USA), and Rifabutin (RFB) from Pharmacy Biotech AB (Uppsala, Sweden). The pure phospholipids, dimyristoyl phosphatidyl choline (DMPC), dimyristoyl phosphatidyl glycerol (DMPG), dipalmitoyl phosphatidyl choline (DPPC), dipalmitoyl phosphatidyl glycerol (DPPG) and dioleoyl phosphatidyl ethanolamine (DOPE) were purchased from Lipoid (Ludwigshafen, Germany). Stearylamine (SA) was obtained from Sigma–Aldrich (St. Louis, MO, USA). Cholesteryl hemisuccinate (CHEMS) and rhodamine covalently linked to phosphatidylethanolamine (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Nuclepore Track-Etch Membranes were purchased from Whatman Ltd. (Maidstone, UK). Thiazolyl Blue Tetrazolium Bromide (MTT) and Crystal Violet (CV) were obtained from Panreac Applichem, ITW Reagents (Darmstadt, Germany). Culture media, Mueller-Hinton Agar (MHA) and Mueller-Hinton Broth (MHB) were obtained from Oxoid, Ltd. (Basingstoke, UK), Tryptic Soy Broth (TSB) from Biokar (Pantin, France) and Columbia Agar + 5% sheep blood (CA) from bioMérieux (Marcy l’Étoile, France). The SYTO 9 was obtained from Molecular Probes (Eugene, OR, USA). All the remaining chemicals used were of analytical grade.
Ferreira M., Pinto S.N., Aires-da-Silva F., Bettencourt A., Aguiar S.I, & Gaspar M.M. (2021). Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics into Staphylococcus aureus Biofilms. Pharmaceutics, 13(3), 321.
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4

Optimizing Irinotecan Delivery in Mice

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Trihydrated irinotecan hydrochloride (IRN) was donated from EUROFARMA Laboratórios SA (São Paulo, Brazil). The dioleoylphosphatidylethanolamine (DOPE), and distearoylphosphatidylethanolamine polyethyleneglycol2000 (DSPE-PEG2000/DSPE-PEG-FOL) were supplied by Lipoid GmbH (USA). Cholesteryl hemisuccinate (CHEMS) and cholesterol (CHOL) were purchased from Sigma-Aldrich Chemical Company (St Louis, USA). Glucose was purchased from Vetec Química Fina Ltda (São Paulo, Brazil). All solvents (high-performance liquid chromatography analytical grade) and other reagents were purchased from Sigma-Aldrich (São Paulo, Brazil). The subcutaneous tumor model was established in 8–10- week-old female BALB/c mice purchased from CEBIO-UFMG (Belo Horizonte, Brazil). Animals were kept under SPF condition with free access to standard food and water. All animal studies were approved by the local Ethics Committee for Animal Experiments of Federal University of Minas Gerais, Brazil (protocol # 102/2020).
Nunes S.S., Miranda S.E., de Oliveira Silva J., Fernandes R.S., de Alcântara Lemos J., de Aguiar Ferreira C., Townsend D.M., Cassali G.D., Oliveira M.C, & de Barros A.L. (2021). pH-responsive and folate-coated liposomes encapsulating irinotecan as an alternative to improve efficacy of colorectal cancer treatment. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 144, 112317.
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5

Radiolabeled Bombesin Peptide Delivery System

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Cholesteryl hemisuccinate (CHEMS), doxorubicin, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) sodium salt, sodium alendronate, sodium hydroxide, and D-luciferin were obtained from Sigma Chemical Company (Saint Louis, MI, USA). Lipoid GmbH (Ludwigshafen, Germany) supplied Dioleoylphosphatidylethanolamine (DOPE) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N- (polyethyleneglycol-2000) (DSPE-PEG2000). Dulbecco's modified Eagle's medium (DMEM) was purchased from Gibco (Grand Island, NY, USA). The human breast adenocarcinoma cell line (MDA-MB-231) was purchased from American Type Culture Collection (Manassas, VA, USA). Xylazine solution (Dopaser™ 2%) was obtained from Hertape Calier (Juatuba, MG, Brazil). Ketamine hydrochloride solution (Dopalen™ 10%) was purchased from Vetbrands Agroline (Campo Grande, MT, Brazil). HYNIC-βAla-Bombesin7–14(99mTc-BBN7–14) peptide was supplied by GL BioChem (Shanghai Shi, China), and technetium-99m was obtained by a molybdenum-99/technetium-99m generator from Instituto de Pesquisas Energéticas (São Paulo, Brazil). All other chemicals used in this study were of analytical grade.
dos Santos Ferreira D., de Oliveira Pinto B.L., Kumar V., Cardoso V.N., Fernandes S.O., Souza C.M., Cassali G.D., Moore A., Sosnovik D., Farrar C., Leite E.A., Alves R.J., de Oliveira M.C., Guimarães A.R, & Caravan P. (2017). EVALUATION OF ANTITUMOR ACTIVITY AND CARDIAC TOXICITY OF A BONE-TARGETED pH-SENSITIVE LIPOSOMAL FORMULATION IN A BONE METASTASIS TUMOR MODEL IN MICE. Nanomedicine : nanotechnology, biology, and medicine, 13(5), 1693-1701.
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