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Cd163 apc

Manufactured by Miltenyi Biotec
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CD163-APC is a fluorochrome-conjugated antibody that specifically binds to the CD163 antigen. CD163 is a scavenger receptor expressed on the surface of monocytes and macrophages. The APC (Allophycocyanin) fluorochrome allows for the detection of CD163-positive cells using flow cytometry.

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Lab products found in correlation

Gentlemacs dissociator, by Miltenyi Biotec (2 mentions) Macsquant analyzer, by Miltenyi Biotec (2 mentions) Macsquantify software, by Miltenyi Biotec (2 mentions) Cd14 percp cy5, by BioLegend (1 mentions) Cd56 percp cy5, by BioLegend (1 mentions) Facsverse flow cytometer, by BD (1 mentions) Cd11b apc, by BioLegend (1 mentions) Cd206, by BioLegend (1 mentions) Anti mouse cd45 vioblue, by Miltenyi Biotec (1 mentions) Macs tissue storage solution, by Miltenyi Biotec (1 mentions) Alexa fluor 488 conjugated mouse anti human cd11b, by R&D Systems (1 mentions) Fab16991g 100, by Miltenyi Biotec (1 mentions) Ic0041g, by R&D Systems (1 mentions)

Close spelling variants of this product

Mouse anti-human CD163-APC Anti-CD163-APC CD163-APC-Vio770 CD163

4 protocols using cd163 apc

1

Multiparametric Flow Cytometry Analysis of GBM

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PBMCs from GBM patients and healthy controls were stained with mAb against human CD3 FITC (Miltenyi Biotec, Bergisch Gladbach, Germany), CD56 PerCP-Cy5.5 (BioLegend, San Diego, CA, USA), NKp44 APC (Miltenyi Biotec, Bergisch Gladbach, Germany), CD11b APC (BioLegend, San Diego, CA, USA), CD14 PerCP Cy5.5 (BioLegend, San Diego, CA, USA), and CD206 (BioLegend, San Diego, CA, USA), CD163 APC (Miltenyi Biotec). The U87-MG cells were stained with mAb against human MDR PerCP Cy5.5 (BioLegend, San Diego, CA, USA). After 30 min incubation at 4 °C in the dark, cells were washed, centrifuged, and resuspended in staining buffer for the FACS analysis. For each test, cells were analyzed using a FACSVerse flow cytometer (BD Biosciences, Swindon, UK). The U87-MG cells (treated as mentioned above), healthy controls, and patients’ PBMCs were also stained for Sa-β-galactosidase and incubated for 15 min at 37 °C in the dark without washing before FACS analysis.
Puca A.A., Lopardo V., Montella F., Di Pietro P., Cesselli D., Rolle I.G., Bulfoni M., Di Sarno V., Iaconetta G., Campiglia P., Vecchione C., Beltrami A.P, & Ciaglia E. (2022). The Longevity-Associated Variant of BPIFB4 Reduces Senescence in Glioma Cells and in Patients’ Lymphocytes Favoring Chemotherapy Efficacy. Cells, 11(2), 294.
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2

Flow Cytometry for Brain Immune Cells

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Fourteen days after IC MDM inoculation, mice were sacrificed and brain samples were harvested and placed in a tissue preservative solution (Miltenyi, Bergisch Gladbach, Germany). Samples were then dissociated using a commercial kit and a gentleMacs dissociator (Miltenyi) into single cell suspensions. Cells were then washed in 2% BSA/PBS buffer and incubated with the following: (1) anti-murine CD45-Vioblue (1:10 dilution) and anti-murine MHCII primary antibody (1:500) followed by APC conjugated secondary antibody (1:500 dilution), or isotype-only controls; (2) GFAP-FITC (1:750 dilution 1′ antibody and 1:1000 2′ 488 (FITC) antibody (Abcam, Cambridge, UK); or (3) human (non-murine cross-reactive) CD163-APC (Miltenyi Biotec) for 30 min (Sigma Aldrich, St. Louis, MO). Cells were washed twice in 2% BSA/PBS and fixed with 2% paraformaldehyde for 15 min. Cells were washed twice in 2% BSA/PBS and analyzed for double positive cells by flow cytometry (Macs Quant analyzer and MacsQuantify software, Miltenyi Biotec).
Gavegnano C., Haile W.B., Hurwitz S., Tao S., Jiang Y., Schinazi R.F, & Tyor W.R. (2019). Baricitinib reverses HIV-associated neurocognitive disorders in a SCID mouse model and reservoir seeding in vitro. Journal of Neuroinflammation, 16, 182.
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3

Characterization of Murine Neuroinflammatory Responses

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Fourteen days after IC MDM inoculation, mice were sacrificed and brain samples were harvested, perfused, and placed in a tissue preservative solution (MACS Tissue Storage Solution, Miltenyi, Bergisch Gladbach, Germany). Samples were then dissociated using a commercial kit and a gentleMacs dissociator (Miltenyi) into single cell suspensions. Cells were then washed in 2% BSA/PBS buffer and incubated with 1) anti-mouse CD45-Vioblue (Miltenyi Biotec San Diego, CA; 1:10 dilution) and anti-mouse MHCII primary antibody (Millipore, Burlington, MA; 1:500) followed by APC conjugated secondary antibody (1:500 dilution), or isotype only controls, 2) GFAP-FITC (Millipore; 1:750 dilution 1’ antibody and 1:1,000 2’ 488 (FITC) antibody (Abcam, Cambridge, UK), or 3) human (non-murine cross-reactive) CD163-APC (Miltenyi Biotec) for 30 minutes (Sigma Aldrich, St. Louis, MO), or 4) MAP-2-FITC (Millipore; 1:250 dilution 1’ antibody and 1:1,000 2’ antibody 488 (FITC). Cells were washed twice in 2% BSA/PBS and fixed with 2% paraformaldehyde for 15 minutes. Cells were washed twice in 2% BSA/PBS and analyzed for double positive cells by flow cytometry (Macs Quant analyzer and MacsQuantify software, Miltenyi Biotec).
Gavegnano C., Haile W., Koneru R., Hurwitz S.J., Kohler J.J., Tyor W.R, & Schinazi R.F. (2020). Novel Method to Quantify Phenotypic Markers of HIV-Associated Neurocognitive Disorder in a Murine SCID Model. Journal of neurovirology, 26(6), 838-845.
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4

Isolation and Identification of Tumor-Associated Macrophages from GBM

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The cells isolated from human GBM tumors were incubated with Alexa Fluor 488-conjugated mouse anti-human CD11b (R&D Systems, Minneapolis, MN; FAB16991G-100) and CD163-APC (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany; 130-100-612) or control immunoglobulin G (IgG) (Miltenyi, 130-098-846 and R&D Systems, IC0041G) for 30 min at 4 °C. FACS was performed to sort TAMs using a BD FACSAria II cell sorter (Becton, Dickinson and Company, NJ).
Zhao G., Ding L., Yu H., Wang W., Wang H., Hu Y., Qin L., Deng G., Xie B., Li G, & Qi L. (2022). M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties. Cell Death Discovery, 8, 350.
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