2',7'-dichlorodihydrofluorescein diacetate was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), RPMI 1600 and an antibiotic solution containing 10,000 units of penicillin, 10 mg of streptomycin and 25 μg of amphotericin B per mL were purchased from Thermo Fisher Scientific, Gibco (Grand Island, NY, USA). BlockAce was purchased from DS Pharma Biomedical (Japan), Biotin-HABP (biotinylated linker protein hyaluronan, 0.25mg/mL) was purchased from Hokudo Co. (Japan), Anti-Human Collagen type 1 antibody was purchased from Rockland Immunochemicals (US), Anti-human MMP-1, anti-IgG/HRP, and Streptavidin-HRP were purchased from R&D Systems (Japan), Can Get Signal was purchased from Toyobo Co. (Japan), EZ West blue dye was purchased from Atto Corp. (Japan), Chlorogenic acid (CGA) was purchased from Cayman Chemical (US). Fluorescein-5-thiosemicarbazide and sodium hyaluronate, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The bicinchoninic acid (BCA) protein assay reagent kit was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA).
Bicinchoninic acid bca protein assay reagent kit
The Bicinchoninic acid (BCA) protein assay reagent kit is a colorimetric detection and quantitation solution used to measure the total protein concentration in a sample. The kit contains reagents that facilitate the reduction of Cu2+ to Cu+ by protein in an alkaline medium, and the subsequent colorimetric detection of the Cu+ cation by bicinchoninic acid.
Lab products found in correlation
3 protocols using bicinchoninic acid bca protein assay reagent kit
Assay for Oxidative Stress and Collagen Degradation
2',7'-dichlorodihydrofluorescein diacetate was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), RPMI 1600 and an antibiotic solution containing 10,000 units of penicillin, 10 mg of streptomycin and 25 μg of amphotericin B per mL were purchased from Thermo Fisher Scientific, Gibco (Grand Island, NY, USA). BlockAce was purchased from DS Pharma Biomedical (Japan), Biotin-HABP (biotinylated linker protein hyaluronan, 0.25mg/mL) was purchased from Hokudo Co. (Japan), Anti-Human Collagen type 1 antibody was purchased from Rockland Immunochemicals (US), Anti-human MMP-1, anti-IgG/HRP, and Streptavidin-HRP were purchased from R&D Systems (Japan), Can Get Signal was purchased from Toyobo Co. (Japan), EZ West blue dye was purchased from Atto Corp. (Japan), Chlorogenic acid (CGA) was purchased from Cayman Chemical (US). Fluorescein-5-thiosemicarbazide and sodium hyaluronate, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The bicinchoninic acid (BCA) protein assay reagent kit was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA).
Optimized CEPO Dosage Administration Protocol
LPS-induced liver injury model
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