Parp 1 antibody

Manufactured by Abcam

Sourced in United States

PARP-1 antibody is a research-use only product that specifically recognizes poly(ADP-ribose) polymerase 1 (PARP-1), a nuclear enzyme involved in DNA repair processes. This antibody can be used for the detection of PARP-1 in various experimental applications such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Aif antibody, by Santa Cruz Biotechnology (1 mentions) Dmi 400cs confocal microscope, by Leica (1 mentions) Decitabine, by Selleck Chemicals (1 mentions) Dnmt1 antibody, by Abcam (1 mentions) Gemcitabine, by Selleck Chemicals (1 mentions) Trametinib, by Selleck Chemicals (1 mentions) P005091, by Selleck Chemicals (1 mentions) Gapdh antibody, by Abcam (1 mentions) Olaparib, by Selleck Chemicals (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Kod plus mutagenesis kit, by Toyobo (1 mentions) Diaminobenzidine dab, by Merck Group (1 mentions) Horseradish peroxidase hrp conjugated goat anti rabbit igg, by Abcam (1 mentions) Fluorescent microscope, by Olympus (1 mentions)

3 protocols using parp 1 antibody

Immunofluorescence Staining of Cochlea and HEI-OC1 Cells

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After being fixed in 4% paraformaldehyde, samples were permeabilized with Triton X-100 (cochlea for 1% and HEI-OC1 cell for 0.2%, Sigma) in PBS for 30 min. Subsequently, specimens were blocked in 10% donkey serum albumin in PBS for 1 h. After 20 h incubation with AIF antibody (1:200, goat polyclonal, Santa Cruz, CA), PARP-1 antibody (1:200, rabbit monoclonal, Abcam, Cambridge, MA) or myosin VIIa antibody (1:800, rabbit polyclonal, CST, US or 1:800, mouse polyclonal, DSHB, Iowa City, IA) at 4 °C, samples were treated with donkey anti-goat, anti-mouse, or anti-rabbit secondary antibodies (1:1000, Life Technologies, Carlsbad, CA) and diamidino-phenyl-indole (DAPI, 1:1000) for 1 h. Then the samples were visualized with an inverted DMI 400CS confocal microscope (Leica, Wetzlar, Germany). Negative controls without primary antibodies had been performed to test the specificity.

Song Y., Fan Z., Bai X., Liu W., Han Y., Xu L., Wang M., Li J., Zheng Q., Zhang D, & Wang H. (2016). PARP-1-modulated AIF translocation is involved in streptomycin-induced cochlear hair cell death. Acta oto-laryngologica, 136(6), 545-550.

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FBP1 Regulation in Cancer Metabolism

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Gemcitabine (#S1714), MK2206 (#S1078), Trametinib (#S2673), Olaparib (#S1060), P005091 (#S7132), Decitabine (# S1200) were purchased from Selleck Chemicals (Shanghai, China). FBP1 antibody (# ab109732, working dilution 1 : 1000), GAPDH antibody (# ab8245, working dilution 1 : 5000), DNMT1 antibody (# ab92314, working dilution 1 : 1000), and PARP1 antibody (# ab32138, working dilution 1 : 1000) were purchased from Abcam. USP7 antibody (# 66514‐1‐Ig, working dilution 1 : 1000) was obtained from Proteintech (Wuhan, China). The KOD‐plus‐mutagenesis kit (#SMK101, TOYOBO LIFE SCIENCE, Osaka, Japan) was used to generate FBP1 mutants mentioned in the results.

Cheng X., Zhang B., Guo F., Wu H, & Jin X. (2021). Deubiquitination of FBP1 by USP7 blocks FBP1–DNMT1 interaction and decreases the sensitivity of pancreatic cancer cells to PARP inhibitors. Molecular Oncology, 16(7), 1591-1607.

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Immunohistochemical Detection of PARP1 in Xenografts

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The expression levels of PARP1 in different xenografts were detected at the protein level by IHC staining. Briefly, xenografts embedded in paraffin were cut into 5 μm sections and fixed onto slides. Then, the slides were deparaffinized, hydrated, and microwave-treated for antigen retrieval. The slides were incubated with the PARP1antibody (1:200 dilution, Abcam, MA, USA) overnight, and then were incubated with secondary antibody, horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:200 dilution, Abcam, MA, USA) at 37° C in dark for 1 hour. Satisfactory immunostaining was acquired in the presence of diaminobenzidine (DAB) (Sigma, MO, USA). Finally, the slides were counterstained with methyl green, subjected to gradient alcohol and xylene dehydration, sealed with neutral gum, and observed under a fluorescent microscope (Olympus, Tokyo, Japan).

Li D., Kou Y., Gao Y., Liu S., Yang P., Hasegawa T., Su R., Guo J, & Li M. (2021). Oxaliplatin induces the PARP1-mediated parthanatos in oral squamous cell carcinoma by increasing production of ROS. Aging (Albany NY), 13(3), 4242-4257.

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