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Aanalyst 100 spectrometer

Manufactured by PerkinElmer
Sourced in United States

The AAnalyst 100 Spectrometer is a versatile, entry-level atomic absorption spectrometer designed for accurate and reliable elemental analysis. It features a robust design, intuitive software, and standard spectral analysis capabilities.

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3 protocols using aanalyst 100 spectrometer

1

Quantifying Total Arsenic in Irrigation Systems

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The total arsenic quantification in the irrigation water, agricultural, soil and the plant parts (root, leaves, pods and seeds) was performed with hydride generation atomic absorption spectrometer (HG-AAS) with continuous flow system FIAS 400 device attached to an AAnalyst 100 spectrometer (PerkinElmer). The mineralization processes of the vegetable matrix and water used in this work are shown in Fig. 1.

Mineralization processes.

Fig. 1
Soil samples were processed for total As according to EPA method 3050 B (1996).
The calibration curve was used in the range of 0,3–5 μg As/L and showed good linearity response of up to 5 μg/L (r = 0.998). The detection and quantification limits was 0.1 and 0.3 μg/L respectively.
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2

Bone Ash Calcium Quantification

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The left tibia of both OVX mice and aged rats were first dried at 110 °C in muffle furnace for 12 h and then incinerated at 800 °C for 20 h. The ash weight was recorded. 100 mg of bone ash was weighed and dissolved in 2 mL of 37% HCl and diluted by Milli-Q water for atomization. The bone calcium content was determined by atomic absorption spectrophotometer (PerkinElmer, AAnalyst 100 Spectrometer, Norwalk, CT, USA).
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3

Quantifying Calcium Metabolism in Bone and Feces

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The left tibia (after micro-CT scanning and with soft tissue removed) and 24 h fecal sample were first dried at 110°C in muffle furnace for 12 h and then ashed at 800°C for 20 h. The ash weight was recorded. To determine the calcium contents, 100 mg of bone/fecal ash was weighed and dissolved overnight in 2 mL of 37% HCl. The samples were diluted with Milli-Q water and analyzed by atomic absorption spectrophotometer (PerkinElmer, AAnalyst 100 Spectrometer, Norwalk, CT, United States). Calcium absorption and balance were calculated by the following equations: Ca absorption rate (%) = (Ca intake - fecal Ca)/Ca intake × 100; Ca balance = Ca intake - (urinary Ca + fecal Ca).
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