Il 6 deficient mice il 6

Kras^LSL-G12D/+, Trp53^LSL-R172H/+, Pdx1-Cre (KPC) mice have been previously described (22 (link)). These mice develop pancreatic intraepithelial neoplasia (PanIN), which progresses to invasive PDAC. C57BL/6 (B6) mice (Jackson Laboratories), IL-6 deficient mice (IL-6^−/−) on the B6 background (Jackson Laboratories) and normal healthy littermate mice (Trp53^LSL-R172H/+, Pdx1-Cre; PC) on the B6 background were used in experiments as controls and for tumor implantation studies. Animal protocols were reviewed and approved by the Institute of Animal Care and Use Committee (IACUC) of the University of Pennsylvania.

C57BL/6J and IL-6-deficient mice (IL-6^−/−) were from Jackson Laboratory (Bar Harbor, ME, USA) and subsequently bred in-house at the Yerkes National Primate Research Center at Emory University or George Washington University. Mice were group housed in ventilated cages and maintained on ad libitum food and water. Lights turned on at 7:00 A.M. and turned off at 9:00 P.M. All experiments were performed between 9:00 A.M. and 5:00 P.M. in 10-16 week-old male mice. Studies were in accordance with NIH guidelines and all procedures were approved by the IACUC at Emory and George Washington University.