Oil red o and hcs lipidtox green neutral lipid staining

Manufactured by Thermo Fisher Scientific

Oil Red O is a fat-soluble dye used to stain neutral lipids in histological samples. HCS LipidTOX Green is a fluorescent dye that selectively stains neutral lipids for high-content screening applications.

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Lab products found in correlation

Stempro adipogenesis differentiation kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

LipidTOX (78 citations) Oil Red O (64 citations) Neutral red (46 citations) LipidTox Red (36 citations) LipidTOX Green (16 citations) HCS LipidTOX Green (10 citations) HCS LipidTox Red (9 citations) HCS LipidTOX (8 citations) LipidTOX Staining (4 citations) LipidTOX Green Neutral Lipid (3 citations)

2 protocols using oil red o and hcs lipidtox green neutral lipid staining

Adipocyte Differentiation Protocol

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Cells were seeded onto 6-well plates at a density of 50,000 cells/well and allowed to recover from trypsinisation for 2 days in maintenance medium. After recovery, cells were cultured in adipocyte differentiation media for 14 days (StemPro Adipogenesis Differentiation Kit, Life Technologies) according to the manufacturer’s instructions. Adipocyte differentiation was evaluated by Oil Red O and HCS LipidTOX Green neutral lipid staining (Invitrogen).

Kennedy E., Mooney C.J., Hakimjavadi R., Fitzpatrick E., Guha S., Collins L.E., Loscher C.E., Morrow D., Redmond E.M, & Cahill P.A. (2014). Adult vascular smooth muscle cells in culture express neural stem cell markers typical of resident multipotent vascular stem cells. Cell and tissue research, 358(1), 203-216.

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Adipocyte Differentiation Evaluation

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Cells were seeded onto 6-well-plates at a density of 50,000 cells/well. Cells were allowed to recover from trypsinisation for 2 days in complete medium. After recovery, cells were cultured in adipocyte differentiation media for 14d (StemPro® Adipogenesis Differentiation, Life Technologies) as according to the manufacturer’s instructions. Adipocyte differentiation was evaluated by Oil Red O and HCS LipidTOX™ Green neutral lipid staining (InVitrogen), as described by the manufacturers protocols.

Kennedy E., Hakimjavadi R., Greene C., Mooney C.J., Fitzpatrick E., Collins L.E., Loscher C.E., Guha S., Morrow D., Redmond E.M, & Cahill P.A. (2014). Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?. Vascular Cell, 6, 6.

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