ICG,
Span 80, and Tween 80 were purchased
from Sigma-Aldrich (St. Louis, MO). Cholesterol was purchased from
LobaChemie (Mumbai, India), and chloroform was purchased from MP Biomedical
LLC. The stock solution of ICG was prepared in deionized (DI) water
(18.2 MΩ Millipore, Sartorius system) and stored at −20
°C. DAPI (2-(4-Amidinophenyl) indole-6-carboxamide-dihydrochloride)
was used for staining the nucleus and was procured from the Tokyo
Chemical Industry (TCI, Shanghai, China). HeLa cells were procured
from the National Centre for Cell Science (NCCS) Pune, India. Dulbecco’s
modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin,
and 0.25% trypsin-1 mM ethylenediaminetetraacetic acid (EDTA) were
purchased from Gibco (Invitrogen).
Span 80, and Tween 80 were purchased
from Sigma-Aldrich (St. Louis, MO). Cholesterol was purchased from
LobaChemie (Mumbai, India), and chloroform was purchased from MP Biomedical
LLC. The stock solution of ICG was prepared in deionized (DI) water
(18.2 MΩ Millipore, Sartorius system) and stored at −20
°C. DAPI (2-(4-Amidinophenyl) indole-6-carboxamide-dihydrochloride)
was used for staining the nucleus and was procured from the Tokyo
Chemical Industry (TCI, Shanghai, China). HeLa cells were procured
from the National Centre for Cell Science (NCCS) Pune, India. Dulbecco’s
modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin,
and 0.25% trypsin-1 mM ethylenediaminetetraacetic acid (EDTA) were
purchased from Gibco (Invitrogen).