Isochrom lc pump

The CHI molecular weight was determined by size exclusion chromatography (SEC) coupled to multi-angle laser light scattering (MALLS). Chitosan solutions at 0.1% (w/v) were prepared in an acetic acid/ammonium acetate buffer pH = 4.5 (AcOH (0.2 M)/AcONH4 (0.15 M)), which was used as eluent. Then, they were filtered through 0.45 μm pore size membranes (Millipore, Merck KGaA, Darmstadt, Germany). The chromatographic equipment was composed of an IsoChrom LC pump (Spectra-Physics, Santa Clara, CA, USA) connected to a Protein Pack 200 SW (Waters GmbH, Eschborn, Germany) column and a TSK gel G6000 PWXL. A multiangle laser light scattering detector DAWN DSP (Wyatt Technology Europe GmbH, Dernbach, Germany) operating at 632.8 nm was coupled online to a WATERS 410 differential refractometer.

The chitosan molecular weight was determined by size exclusion chromatography (SEC) coupled to multi-angle laser light scattering (MALLS) [43 (link)]. Chitosan solution at 0.1% (w/v) was prepared in an acetic acid/ammonium acetate buffer $\mathrm{pH} =4.5$ (AcOH (0.2 M)/AcONH4 (0.15 M)), which was used as eluent. Before SEC measurements, the solution was filtered through 0.45 μm pore size membranes (Millipore). The chromatographic equipment was composed of an IsoChrom LC pump (Spectra-Physics, Charbonnières les Bains, France) connected to a Protein Pack 200 SW column (WATERS, Saint-Quentin-en-Yvelines, France) and a TSK gel G6000 PWXL column (Merck, Saint-Quentin-Fallavier France). A Multi-Angle Laser Light Scattering (MALLS) detector DAWN DSP (Wyatt Technology, Toulouse France) operating at 632.8 nm was coupled on line to a 410 differential refractometer from WATERS (Saint-Quentin-en-Yvelines, France).