Autoanalyzer

Manufactured by Olympus

Sourced in Japan

The Olympus Autoanalyzer is a laboratory instrument designed for automated chemical analysis. It performs high-throughput analysis of a variety of sample types, including biological fluids, environmental samples, and industrial materials. The Autoanalyzer employs various analytical techniques, such as colorimetry, ion-selective electrodes, and enzymatic reactions, to provide accurate and reproducible results.

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Lab products found in correlation

Au400, by Olympus (1 mentions) Bun and scr test kits, by Nanjing Jiancheng (1 mentions) Onetouch ultra, by LifeScan (1 mentions)

Close spelling variants of this product

AU5800 autoanalyzer AU 5400 Autoanalyzer Olympus 5431 autoanalyzer AU2700 autoanalyzer AU400 autoanalyzer

9 protocols using autoanalyzer

Biochemical Markers for Metabolic Assessment

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Fasting blood glucose (FBG) was determined immediately on Olympus auto analyzer using hexokinase method. Serum GOT, GPT, urea, creatinine, uric acid (UA), cholesterol, triglycerides (TG) were determined using colorimetric methods on Olympus AU 400 supplied from Olympus Life and Material Science (Europe GmbH, Wendenstraße, Hamburg, Germany).
TNF-α was determined by ELISA [11 ] kit supplied from Anogen (2355 Derry Road East, Unit 23, Mississauga, Ontario, Canada). Il-6 was determined by ELISA [12 (link)] kit supplied from AviBion (Ani Biotech, tiilitie, Finland). Quantitative determination of hsCRP in serum is done by a micro plate immuoezymometric assay [13 ] supplied from Monobind Inc. (100 North Pointe drive, Lake Forest, USA).

Ismail L.A., Ibrahim A.A., Abdel-Latif G.A., El-Haleem D.A., Helmy G., Labib L.M, & El-Masry M.K. (2015). Effect of Acupuncture on Body Weight Reduction and Inflammatory Mediators in Egyptian Obese Patients. Open Access Macedonian Journal of Medical Sciences, 3(1), 85-90.

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Automated Serum Transaminase Assay

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Human and Experimental Toxicology 40(12S)
Chemistry (IFCC) using pyridoxal phosphate and NADH as cofactors. An Olympus autoanalyzer and commercial kits of the same brand were used for two analyses in the routine biochemistry laboratories of Turgut Ozal Medical Center.
AST and ALT levels were expressed as units per liter.

Caliskan A.R., Gul M., Yılmaz I., Otlu B., Uremis N., Uremis M.M., Kilicaslan I., Gul S., Tikici D., Saglam O., Yalcin M., Demirel U, & Harputluoglu M. (2021). Effects of larazotide acetate, a tight junction regulator, on the liver and intestinal damage in acute liver failure in rats. Human & experimental toxicology, 40(12_suppl).

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Serum Creatinine and Blood Urea Nitrogen Measurement

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Serum creatinine (Scr) and blood urea nitrogen (BUN) were measured by an Auto Analyzer (Olympus, Optical Co. Ltd., Tokyo, Japan) with BUN and Scr test kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), following the manufacturer's instructions.

Yuzeng Q., Weiyang H., Xin G., Qingson Z., Youlin K, & Ke R. (2014). Effects of Transplantation with Marrow-Derived Mesenchymal Stem Cells Modified with Survivin on Renal Ischemia-Reperfusion Injury in Mice. Yonsei Medical Journal, 55(4), 1130-1137.

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Biochemical Markers in Clinical Assessment

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Serum bilirubin, aspartate transaminase, alanine transaminase, alkaline phosphatase, and serum albumin levels were estimated by Olympus autoanalyzer in the Department of Pathology, G.S.V.M. Medical College, Kanpur.

Jain A., Jain S., & Kothari N. (2022). AN EXPERIMENTAL STUDY PERFORMED TO COMPARE THE HEPATOPROTECTIVE ACTIVITY OF ALOE VERA AND SILYMARIN IN CARBON TETRA CHLORIDE (CCL4)-INDUCED HEPATOTOXICITY IN ALBINO RABBITS. Asian Journal of Pharmaceutical and Clinical Research.

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Nephrotoxicity Biomarker Quantification

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Using standard laboratory procedures, BUN and Cr levels were determined to analyze nephrotoxicity by commercial kits with an autoanalyzer (Olympus Instruments, Tokyo, Japan).

Sadeghi H., Karimizadeh E., Sadeghi H., Panahi kokhdan E., Mansourian M., Abbaszadeh-Goudarzi K., Shokripour M., Asfaram A, & Doustimotlagh A.H. (2021). Protective Effects of Hydroalcoholic Extract of Rosa canina Fruit on Vancomycin-Induced Nephrotoxicity in Rats. Journal of Toxicology, 2021, 5525714.

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Plasma Magnesium Concentration Analysis

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Plasma Mg concentration was measured by a colorimetric method using an Olympus Autoanalyzer. A plasma Mg concentration of 0.75 to 0.95 mmol/L (1.8 to 2.3 mg/dL) was used as the normal range for clinical diagnostic purposes [1 (link)]. Therefore, three groups were defined as follows: low (<0.75 mmol/L), normal (0.75–0.95 mmol/L), and high (>0.95 mmol/L). The plasma glucose, serum C-reactive protein (CRP), and serum creatinine concentrations were also measured at baseline to establish histories of diabetes, inflammatory disorders, and impaired kidney function.

Huang Y.C., Wahlqvist M.L., Kao M.D., Wang J.L, & Lee M.S. (2015). Optimal Dietary and Plasma Magnesium Statuses Depend on Dietary Quality for a Reduction in the Risk of All-Cause Mortality in Older Adults. Nutrients, 7(7), 5664-5683.

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Fasting Lipid Profile Measurement

Cited 2 times

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Fasting blood samples were collected in the morning after at least 12 h of fasting for all participants. Blood samples were obtained from an antecubital vein and collected in Vacutainer tubes containing ethylenediaminetetraacetic acid (EDTA). Values for fasting plasma glucose (FPG), TC, LDL-C, HDL-C, TG, and other routine blood biochemical indexes were obtained using an autoanalyzer (Olympus, Kobe, Japan). According to the National Cholesterol Education Program-Third Adult Treatment Panel (ATP III) criteria, high TC was defined as TC ≥ 6.21 mmol/L (240 mg/dL); low HDL-C was defined as HDL-C < 1.03 mmol/L (40 mg/dL); high LDL-C was defined as LDL-C ≥ 4.16 mmol/L (160 mg/dL); and high TG was defined as ≥2.26 mmol/L (200 mg/dL) [14 (link)]. Dyslipidemia was defined as having at least one high LDL-C, high TC, low HDL-C, or high TG, as this method had been widely used in China [15 (link),16 (link)].

Zhang N., Chen Y., Chen S., Jia P., Guo X., Sun G, & Sun Y. (2017). Self-Reported Snoring Is Associated with Dyslipidemia, High Total Cholesterol, and High Low-Density Lipoprotein Cholesterol in Obesity: A Cross-Sectional Study from a Rural Area of China. International Journal of Environmental Research and Public Health, 14(1), 86.

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Kidney Oxidative Stress Assessment

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Kidneys were homogenized in ice-cold phosphate buffer (pH 7.4) to produce 10% homogenate. Tissues were homogenized in a motor-driven tissue homogenizer (IKA Ultra-Turrax T25 Basic; Labortechnic, Staufen, Germany) and sonicator (UW–2070 Bandelin Electronic, Germany) with phosphate buffer (pH 7.4). Unbroken cells, nuclei and cell debris were sedimented by centrifugation at 10000g for 10 min at 4 °C. Protein levels in the homogenate were determined according to the method of Bradford et al. [18 (link)]. This tissue homogenate was used for to determination of TAS and TOS levels [19 (link),20 (link)]. The TAS levels of samples were measured spectrophotometrically at the 660 nm absorbance. The results were expressed as mmol Trolox Eq/mg protein. The color intensity is related to the total amount of oxidant (TOS) molecules in the samples. The results are expressed in terms of mM hydrogen peroxide equivalent per g liter (mmol H2O2 Equiv/L, mmol H₂O₂ Equiv/ mg protein). Determination of OSI, which is an indicative parameter of oxidative stress level and the ratio of TOS to TAS was calculated using the following formula [21 (link)]:
TAS and TOS were measured by the automated chemistry analyzer Beckman Coulter AU5800 (Japan). Serum BUN, uric acid and creatinine levels were determined using the Olympus AutoAnalyzer (Olympus Instruments, Tokyo, Japan) and results are expressed as mg/dl.

Asci H., Ozmen O., Ellidag H.Y., Aydin B., Bas E, & Yilmaz N. (2017). The impact of gallic acid on the methotrexate-induced kidney damage in rats. Journal of Food and Drug Analysis, 25(4), 890-897.

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Plasma Biomarker Analysis Protocol

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Plasma glucose concentrations were determined using a glucose analyzer (OneTouch® Ultra™, Lifescan, Inc., Milpitas, CA, USA) at the experimental end-point. Plasma total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c) and triglyceride (TG) concentrations were determined by enzymatic colorimetry in an Olympus® auto-analyzer (Olympus America, Inc., NY, USA) resorting to commercial kits (OSR6516, OSR6587, and OSR61118, Olympus America, Inc., for TC, HDL-c and TG, respectively). Low-density lipoprotein cholesterol (LDL-c) levels were calculated by the formula of Friedewald (LDL-c = TC − [HDL-c − 1/5 TG], if serum TG is 400 mgdL^− 1 or less) [21 (link)].
C-reactive protein (CRP) levels in serum were analyzed with an immunoturbidimetric latex CRP assay, normal set (OSR6199, Olympus America, Inc.).

Rocha B., Rodrigues A.R., Tomada I., Martins M.J., Guimarães J.T., Gouveia A.M., Almeida H, & Neves D. (2018). Energy restriction, exercise and atorvastatin treatment improve endothelial dysfunction and inhibit miRNA-155 in the erectile tissue of the aged rat.. Nutrition & Metabolism, 15, 28.

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