HR-SEM analysis was done for Fmoc–DOPA–DOPA hydrogels at different time points following the initiation of peptide self-assembly. Then, 10 μL samples were dried at room temperature on microscope glass coverslips and coated with chromium. Images were taken using a JEOL JSM 6700F FE-SEM operating at 10 kV.
Jsm 6700f fe sem
Manufactured by JEOL
Sourced in Japan
The JSM-6700F is a field emission scanning electron microscope (FE-SEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of a wide range of materials and samples. The JSM-6700F utilizes a field emission electron source to generate a high-brightness, high-resolution electron beam, enabling detailed examination of small-scale features and structures.
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15 protocols using jsm 6700f fe sem
1
Time-Lapse Fmoc-DOPA-DOPA Hydrogel Imaging
2
Characterization of Iron Oxide Nanoparticles
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The morphology of the iron oxide nanoparticles (IONPs) was determined by X-ray diffractometer (Bruker D8 advance) using CuKα radiation ranging from 20°-80°. The size and shape of the IONPs were assessed through scanning electron microscopy (FE-SEM, HR-TEM) images. The SEM images were recorded using a microscope in JSM-6700F FESEM, JEOL, Japan and TEM images were obtained on a JEM-2100 HRTEM, JEOL, Japan.
3
Scanning Electron Microscopy of Implants
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Rabbits were euthanized on post-operative days 28 and 56 for SEM, as previously described (Pribaz et al., 2012 (link)). Briefly, the ex vivo implants were carefully extracted as not to disturb the surface structures and were immediately fixed in buffered formaldehyde (4%) and glutaraldehyde (2.5%) for 48 h and rinsed with buffer thrice (10 min each). Secondary fixation was performed for 1 h with osmium tetroxide (1%) in PBS. Dehydration was carried out by incubating the implants (15 min each) in increasing concentrations of ethanol (30%, 50%, 67%, 80%, 90%, 100% and 100%). Subsequently, the implants were immersed in decreasing mixtures of ethanol and hexamethyldisilazane (HMDS) (ethanol:HMDS=3:1, 1:1 and 1:3) followed by 100% HMDS dehydration twice (15 min each). Samples were air-dried overnight to ensure evaporation of HMDS. All implants were mounted on aluminum stub mounts, sputter-coated with gold-palladium prior to imaging under a field-emission scanning electron microscope (JSM-6700F FE-SEM; JEOL, Tokyo, Japan) at 35×, 70×, 350× and 1500× magnification.
4
Structural and Optoelectronic Characterization of PbI2 Microplates
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The structure of PbI2 microplate arrays was characterized using an optical microscope (Olympus), a scanning electron microscope (JSM-6700F FE-SEM; JEOL), and an x-ray diffractometer (PANalytical X’Pert Pro). Photoluminescence was measured on a confocal micro-Raman system (LabHR; HORIBA) in backscattering configuration excited by an argon-ion laser (488 nm) with 1.5 μW of excitation power. Photoresponse was measured on the same micro-Raman system coupled with a computer-controlled analog-to-digital converter (model 6030E; National Instruments). The photoresponse of the U-shaped array was measured in a probe station (TTP4; LakeShore) coupled with a computer-controlled analog-to-digital converter (model 6030E; National Instruments) and illuminated by a blue LED with a peak wavelength of 463 nm and a power density of 600 μW/cm2. For measurement of response speed, a mechanical chopper was used to modulate the incident light. FET device measurements were performed in a probe station (TTP4; LakeShore) coupled with a precision source/measurement unit (B2902A; Agilent Technologies). The scanning rate for transport measurement was 20 V/s, and the devices were prebiased at the opposite voltage for 30 s before each measurement.
5
HRSEM Imaging of Sample Coatings
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HRSEM imaging was performed by applying 5 μl solution samples on glass coverslips, allowing them to dry under ambient conditions overnight and coating the samples with Cr. Micrographs were recorded using a JSM-6700F FE-SEM (JEOL, Tokyo, Japan) operating at 2 kV.
6
Imaging Fmoc-DOPA Self-Assemblies
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To image solution samples, 5-μl samples of Fmoc-DOPA (1 mg/ml), taken at different time points, were dried at room temperature on microscope glass coverslips. To image the powder of lyophilized Fmoc-DOPA assemblies, samples of Fmoc-DOPA (1 mg/ml), taken at different time points, were flash-frozen in liquid nitrogen and lyophilized for at least 20 hours. All samples were coated with approximately 30 nm of Cr. Images were taken using a JEOL JSM-6700F FE-SEM operating at 10 kV.
7
Graphene-Based Antithrombotic Biomaterials
8
Fmoc-G-PNA Conjugate Self-Assembly
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The Fmoc-G-PNA conjugate was dissolved in double distilled water to a concentration of 0.1 mg/mL by heating the solution to 100 °C under continuous stirring. The partially dissolved hot solution was filtered using a 0.22 μm PVDF syringe filter (Millipore) into a clean beaker with a glass cover slip inside. The turbidity of the solution increased within several minutes as the solution cooled to room temperature, indicating self-assembly. Excess solution was drawn out gently using a syringe. The glass was dried in open air and images were taken using a JEOL JSM 6700F FE-SEM operating at 10 kV, after coating with Cr.
9
Amino Acid SEM Imaging Protocol
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The amino acids
were dissolved in deionized water at a concentration of 2 mg/mL by
heating to 90 °C followed by gradual cooling of the solutions.
Fordl -composites, 1 mg of each amino acids was similarly
dissolved in 1 mL of deionized water. A 5 μL aliquot was allowed
to dry on a microscope glass coverslip at ambient conditions overnight
and coated with Au. SEM images were recorded using a JSM-6700F FE-SEM
(JEOL, Tokyo, Japan) operating at 10 kV.
were dissolved in deionized water at a concentration of 2 mg/mL by
heating to 90 °C followed by gradual cooling of the solutions.
For
dissolved in 1 mL of deionized water. A 5 μL aliquot was allowed
to dry on a microscope glass coverslip at ambient conditions overnight
and coated with Au. SEM images were recorded using a JSM-6700F FE-SEM
(JEOL, Tokyo, Japan) operating at 10 kV.
10
Polymer Sample Preparation for SEM
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SEM images were taken by the Molecular Instrumentation Center at UCLA’s Department of Chemistry and Biochemistry. Briefly, plastic samples were sequentially washed with 70% EtOH, 1% SDS in ddH2O, and ddH2O, and air dried. The samples were coated with an 8 nm gold layer, and images were acquired using a JEOL JSM-6700F FE-SEM using the LEI detector.
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