The breast cancer cell lines 4T1, MDA-MB-231, BT549 and HEK 293 human kidney cells were obtained from the American Type Culture Collection (ATCC®). 4T1, MDA-MB-231 and HEK 293 cells were cultured in high glucose DMEM (Gibco®) supplemented with 10% FBS (Gibco®). BT549 cells were cultured in RPMI-1640 (Gibco®) supplemented with 10% FBS. All cells were maintained at 37°C in a humidified 5% CO2 atmosphere. The ALK5 inhibitor A8301, HDAC8 inhibitor PCI-34051 and paclitaxel were obtained from MedChemExpress (MCE®). Other HDAC inhibitors mentioned in manuscript were from Selleck.
Pci 34051
Manufactured by MedChemExpress
Sourced in Sweden, United States
PCI-34051 is a laboratory equipment product manufactured by MedChemExpress. It is a small molecule that functions as a potent and selective inhibitor of the epidermal growth factor receptor (EGFR) tyrosine kinase.
Automatically generated - may contain errors
Lab products found in correlation
Romidepsin, by MedChemExpress (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rgfp966, by MedChemExpress (2 mentions)
Bt 549, by Thermo Fisher Scientific (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
A83 01, by MedChemExpress (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Hek293, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Bt549, by American Type Culture Collection (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Paclitaxel, by MedChemExpress (1 mentions)
Decitabine, by MedChemExpress (1 mentions)
Itsa 1, by MedChemExpress (1 mentions)
Romidepsin, by R&D Systems (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Endothelial growth medium 2, by Lonza (1 mentions)
Tubacin, by MedChemExpress (1 mentions)
Tazemetostat, by MedChemExpress (1 mentions)
4 protocols using pci 34051
1
Breast Cancer Cell Line Culture Protocols
2
Endothelial Cell HDAC Modulation by Romidepsin
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Primary HAEC were purchased from the American Type Culture Collection (Catalog No. PCS-100-011, Lot no. 63233442, Manassas, VA) and maintained in Endothelial Growth Medium-2 (Lonza) supplemented with 10% FBS (GIBCO). HDAC inhibitor Romidepsin (HY-15149), RGFP966 (HY-13909) and PCI-34051 (HY-15224), HDAC activator ITSA-1 (HY-100508) and DNMT inhibitor Decitabine (5-Aza-2'-deoxycytidine, DCA, HY-A0004) were products of MedChemExpress. Unless otherwise indicated, HAEC up to passage 10 without serum starvation were pretreated with Romidepsin (40 nM) for 1 h and then stimulated with 0.1 ng/mL TNFα (R&D Systems) for 2 h for mRNA measurement, or for 4 h for Western blot and flow cytometry analyses.
3
Evaluation of Pharmacological Inhibitors
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Recombinant human NT-3 was obtained from ImmunoTools (Friesoythe, Germany). Entinostat and PD 98,059 were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Vorinostat, romidepsin, PCI-34051, tubacin, 3-deazaneplanocin A hydrochloride, (+)-JQ-1 and tazemetostat were obtained from MedChem Express Europe (Sollentuna, Sweden). VPA and 4′,6-diamidino-2phenylindole dihydrochloride (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). BIRB 0796 was obtained from Axon Medchem BV (Groningen, The Netherlands), whereas TCS JNK6o was from Tocris Bioscience (Bristol, UK).
4
Epigenetic Mechanisms in B7x Regulation
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The Pan-HDAC inhibitor SAHA, the HDAC1,2 inhibitor Romidepsin, the HDAC3 inhibitor RGFP966, the HDAC1,3 inhibitor ITF-2357, the HDAC6 inhibitor ACY-775 and the HDAC8 inhibitor PCI-34051 were obtained from MedChemExpress, USA. The primary antibodies against HDAC3, NF-κB, Lef-1, C/EBP-α, PARP, and β-actin were purchased from Cell Signaling Technology (Danvers, MA). The antibody B7x and HDAC1 used for immunohistochemistry were got from Cell Signaling Technology (Danvers, MA) and Sant Cruz Technology (Dallas, TX), respectively. The primary antibody against RFX-1 was obtained from Abcam Technology (Cambridge, MA). The mouse B7x antibody was generated by our lab (Albert Einstein College of Medicine). The HDAC siRNAs and C/EBP-α siRNA was from Life Technologies, USA.
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