Lymphatic-specific Prox1-EGFP reporter mice (FVB background)61 (link) were purchased from Mutant Mouse Regional Resource Centers, FVB/NCrl mice were purchased from Charles River Laboratories, and C57BL/6 mice were purchased from Jackson Laboratory. Mice with inducible deletion of PDPN (Pdpnf/f;CagCre) and WT littermates (Pdpnf/w;CagCre) in mixed background (C57BL/6 and 129/Sv) were generated as previously described49 (link). PDPN deletion was accomplished by administering tamoxifen orally (20 μg each day) from P1 to P6. After weaning, the mice were orally administered 1 mg tamoxifen weekly. The Lgals8 KO mouse strain used for this study was created from embryonic stem cell clone (14305A-F8), obtained from the KOMP Repository (www.komp.org ) and generated by Regeneron Pharmaceuticals, Inc62 (link). The Lgals8 coding region deletion was achieved by LacZ (bacterial β-galactosidase) reporter gene replacement of chromosome 13 from 12,440,786 (deletion start) to 12,459,212 (deletion end). Details of the primer sequences and predicted PCR products are available at the Velocigene website (www.velocigene.com/komp/detail/14305 ). The galectin-8 null status of the KO mice is confirmed by western blotting (Supplementary Fig. 11 ). The Lgals8 KO mice have no obvious defects in lymphatic vessel development examined by gross morphological analysis.
Fvb ncrl mice
Manufactured by Charles River Laboratories
Sourced in United States
FVB/NCrl mice are an inbred laboratory mouse strain. They are characterized by their white coat color, black eyes, and susceptibility to developing spontaneous retinal degeneration. The strain is commonly used in genetic research and transgenic studies.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6ntac, by Taconic Biosciences (2 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
B6 cg tg krt1 15 egfp 2cot j, by Jackson ImmunoResearch (1 mentions)
Clone rmp1 14, by BioXCell (1 mentions)
Balb c, by Charles River Laboratories (1 mentions)
Clone 10f 9g2, by BioXCell (1 mentions)
129 e mice, by Charles River Laboratories (1 mentions)
10 protocols using fvb ncrl mice
1
Generation and Characterization of Lymphatic and PDPN Knockout Mouse Models
2
Generation and Characterization of En1 Knockout Mice
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CD1 (Crl:CD1) timed pregnant mice and FVB/NCrl mice were purchased from Charles River Laboratories. C57BL/6J mice were purchased from the Jackson Laboratories. En1KO mice were generated in the laboratory of Alexandra Joyner (35 (link)) and were obtained from the laboratory of Susan Dymecki. En1KO mice were bred onto C57BL/6NTac (Taconic Biosciences) for at least 10 generations. All experiments were performed in accordance with approved Institutional Animal Care and Use Committee (IACUC) protocols.
3
AREG Expression Constructs in pBK5 Vector
4
FVB/NCrl Mice Animal Studies
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Animal studies were approved by both Creighton University and Boys Town National Research Hospital Institutional Animal Care and Use Committees and were consistent with the National Research Council Guide for the Care and Use of Laboratory Animals (2011). FVB/NCrl mice were purchased from Charles River Laboratories.
5
FVB/NCrl Mice Acclimatization and Experiments
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Female and male FVB/NCrl mice (8–10 weeks old, Charles River Stock No. 207) were acclimatized and housed at the Coro Building Barrier facility. Experiments were carried out in accordance with the approved protocol via the Institutional Animal Care and Use Committee (Protocol 1844667/CMTT# 5017–22).
6
Two-stage Carcinogenesis Protocol for Skin Papilloma
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All animal experiments were approved by the NC State University Institutional Animal Care and Use Committee (IACUC). FVB/NCrl mice were obtained from Charles River laboratories (strain 207) and K15EGFP mice from The Jackson Laboratory (B6.Cg-Tg [Krt1-15-EGFP] 2Cot/J, Stock number 005244).
Skin papillomas were induced in transgenic mice overexpressing CDK4 in the epidermis (K5CDK4) by the two-stage carcinogenesis protocol as we previously described (27 (link),28 (link)). Briefly, groups of 20 mice each (K5CDK4 and wild type siblings) were initiated at day 1 after birth by topical application of 50 mg of DMBA in 50 ml of acetone on the mouse back. At day 14, mice received 2.5 mg of TPA in 200 ml of acetone twice a week for 20 weeks. Skin papillomas were collected at the end of the experiment at 30 weeks. Immunohistochemical analysis of skin tumors was performed on paraffin-embedded sections from mouse skin papillomas as described below.
Skin papillomas were induced in transgenic mice overexpressing CDK4 in the epidermis (K5CDK4) by the two-stage carcinogenesis protocol as we previously described (27 (link),28 (link)). Briefly, groups of 20 mice each (K5CDK4 and wild type siblings) were initiated at day 1 after birth by topical application of 50 mg of DMBA in 50 ml of acetone on the mouse back. At day 14, mice received 2.5 mg of TPA in 200 ml of acetone twice a week for 20 weeks. Skin papillomas were collected at the end of the experiment at 30 weeks. Immunohistochemical analysis of skin tumors was performed on paraffin-embedded sections from mouse skin papillomas as described below.
7
Transgenic Mice Model for Cancer Research
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MMTV-PyMT transgenic mice were backcrossed with FvB/NCrl mice (Charles River laboratories). Ifnar−/− mice were kindly provided by A. Lebon, Institut Cochin, Paris, France. Mice were maintained at the Cochin Institute SPF animal facility. Animal care was performed by expert technicians in compliance with the Federation of European Laboratory Animal science association and under the approval of the animal experimentation ethics committee of Paris Descartes (CEEA 34, 16–063).
8
FVB/NCrl Mouse MSC-EVs Tracking
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Female and male FVB/NCrl mice (6–8 weeks old) from Charles River (Stock No. 207) were used in this study, with an n = 5 per experimental group. The animals were housed at the Coro Building Barrier facility, acclimatized appropriately and fed a normal diet. All experimental procedures carried out in accordance with the protocol approved by Institutional Animal Care and Use Committee (Protocol 1844667/CMTT# 5017‐22). The experiments were carried out over several weeks by a single experienced surgeon with freshly prepared fluorescent‐labeled MSC‐EVs or DiD‐saline.
9
Tumor Immunotherapy in Mouse Models
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FVB/NCrl mice, 129-E mice and BALB/c were purchased from Charles River Laboratories. Tumor cells were s.c.-implanted in 7-9-week-old female mice at optimal doses as indicated in figure legends. Tumor length and width were measured by caliper and the volume calculated by the equation: 0.4∗length∗widthˆ2. For immunotherapy studies, mice bearing ∼50mm3 tumors were randomized and treated with genetically engineered DCs as detailed below, anti-PD-1 antibody (BioXcell, Clone RMP1-14), or anti-PD-L1 antibody (BioXcell, Clone 10F.9G2). In tumor re-challenge studies, mice were euthanized when tumor volume reached 1500 mm3. Mice were housed in pathogen-free facilities at UCLA and all procedures were approved by the UCLA Animal Research Committee (ARC protocol # 2017-049).
10
Mouse Breeding and Maintenance for Research
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CD1 (Crl:CD1) timed pregnant and FVB/NCrl mice were purchased from Charles River Laboratories. C57BL/6J mice were purchased from the Jackson Laboratories. En1KO mice were generated in the lab of Dr. Alexandra Joyner [26 (link)] and were obtained from the laboratory of Susan Dymecki (Harvard Medical School). En1KO mice were bred onto C57BL/6NTac (Taconic Biosciences) for at least 10 generations. Mice were housed in a vivarium on a 12h light/dark cycle. See also Table 1 .
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