An HPLC system assembled with a Jasco 880-PU Intelligent pump, a Jasco AS-2055 plus Intelligent Sampler, a Jasco 875-UV Intelligent UV–vis Diodarray detector set at 206 nm (Alexandria, VA, USA), and Borwin v 1.2160 chromatography software were used to determine the toluene concentration in the solutions resulting from adsorption kinetics and isotherm trials. A C18 Luna 5 µm HILIC 150 mm analytical column (Phenomenex, Torrance, CA, USA) was kept in a column oven at 35 °C (Jones Chromatography model 7971) and eluted with a mixture of HPLC grade H2O and CH3CN (45 and 55% by volume, respectively) at 1 mL min−1 of flow rate. Under these chromatographic conditions, the retention time of toluene was 4.3 min. The toluene quantitative determination of toluene, measured as an average value of triplicate injections, was performed by using external standard (rworking curve = 0.99999).
As 2055 plus intelligent sampler
Manufactured by Jasco
Sourced in United States, Japan
The AS-2055 plus Intelligent Sampler is a laboratory equipment product. It is designed to collect and handle samples for analysis.
Automatically generated - may contain errors
Lab products found in correlation
Accq fluor reagent kit, by Waters Corporation (2 mentions)
Intelligent fluorescence detector fp 1520, by Jasco (2 mentions)
Pu 2080 plus intelligent hplc pump, by Jasco (1 mentions)
Accq tag eluent, by Waters Corporation (1 mentions)
Accq tag eluent a concentrate, by Waters Corporation (1 mentions)
Accq fluor reagent, by Waters Corporation (1 mentions)
Pu 1580 intelligent hplc pump, by Jasco (1 mentions)
Accq fluor borate buffer, by Waters Corporation (1 mentions)
4 protocols using as 2055 plus intelligent sampler
1
HPLC Quantification of Toluene in Adsorption Experiments
2
Sialic Acid Quantification by Fluorescence Spectroscopy
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To measure the sialic acid concentration, 10 µL of labeled sample was injected into the device. A LiChroCART®250-4 LiChrospher®100 RP-18e (5 µm) column (Merck KGaA, Darmstadt, Germany) was used. The solvent consisted of a mixture of 86% water, 8% acetonitrile, and 6% methanol, and was used with a flow of 0.6 mL/min. The wavelength chosen for excitation was 373 nm, resulting in an emission at 448 nm. The concentrations were determined using a diluted standard of the according sialic acid (0.5–50 µmol/L, Thermo Fisher Scientific, Waltham, MA, US). The devices used are listed below: Jasco FP-2020 Plus Intelligent Fluorescence Detector, Jasco PU-2080 Plus Intelligent HPLC-Pump, Jasco LG-2080 Plus Quaternary Gradient Unit, Jasco DG-2080-54 4 Line Degasser, Jasco AS-2055 Plus Intelligent Sampler.
3
Amino Acid Profiling by HPLC
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To evaluate the variation in the amino acid concentration, samples were analyzed by HPLC (PU-1580 Intelligent HPLC, Intelligent Fluorescence Detector FP-1520 and Intelligent Sampler AS-2055 Plus, with 10 μL loop; Jasco Corp., Tokyo, Japan), after a derivatization using an AccQ-Fluor Reagent kit (Waters Corp., Milford, MA, USA) according to the method described by Montanari et al. [6 (link)].
The separation of amino acids was performed using an AccQ-TagTM column (3.9 × 150 mm; Waters Corp.) at 30 °C using mobile phase A (100 mL of AccQ-Tag Eluent (Waters Corp., Milford, MA, USA), diluted 1:10 with H2O for chromatography (Sigma-Aldrich, St. Louis, MO, USA) and mobile phase B (60% acetonitrile and 40% H2O for chromatography (Sigma-Aldrich, St. Louis, MO, USA)) at a flow rate of 1 mL/min. The fluorescent detector was set at an excitation wavelength of 250 nm and emission wavelength of 395 nm. Under the adopted conditions, good separation of the amino acids was obtained with the exception of the couples histidine + glutamine and serine + asparagine, which coeluted in unique peaks. Tryptophan was not detectable with this protocol.
The separation of amino acids was performed using an AccQ-TagTM column (3.9 × 150 mm; Waters Corp.) at 30 °C using mobile phase A (100 mL of AccQ-Tag Eluent (Waters Corp., Milford, MA, USA), diluted 1:10 with H2O for chromatography (Sigma-Aldrich, St. Louis, MO, USA) and mobile phase B (60% acetonitrile and 40% H2O for chromatography (Sigma-Aldrich, St. Louis, MO, USA)) at a flow rate of 1 mL/min. The fluorescent detector was set at an excitation wavelength of 250 nm and emission wavelength of 395 nm. Under the adopted conditions, good separation of the amino acids was obtained with the exception of the couples histidine + glutamine and serine + asparagine, which coeluted in unique peaks. Tryptophan was not detectable with this protocol.
4
Amino Acid Analysis of DM Media
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For the variation of amino acid concentrations in DM media, samples were subjected to an AccQ-Fluor Reagent (AQC, 6-aminoquinolyl-N-hydroxysuccinimide carbamate) derivatization (Waters Corp., Milford, MA, United States) according to the manufacturer’s protocol. AQC was reconstituted at a final concentration of 10 mM in acetonitrile, included in the AccQ-Fluor Reagent Kit (Waters Corp.). Briefly, 10 μL of samples were derivatizated with 70 μL of AccQ-Fluor Borate Buffer (Waters Corp.) and 20 μL of reconstituted reagent. The samples were heated to 55°C for 10 min. The amino acids content was analyzed using an HPLC (PU-1580 Intelligent HPLC pump, Intelligent Fluorescence Detector FP-1520 and Intelligent Sampler AS-2055 Plus, with 10 μl loop; Jasco Corp.). Separation of amino acids was obtained using AccQ-TagTM column (3.9 mm × 150 mm) for amino acid analysis (Waters Corp.). A gradient elution was performed maintaining a column temperature of 30°C and using two mobile phases: A (100 ml of AccQ-Tag Eluent A concentrate (Waters Corp.), diluted 1:10 with H2O for chromatography (Sigma-Aldrich, St. Louis, MO, United States) and B (60% acetonitrile and 40% H2O for chromatography) (Sigma-Aldrich, St. Louis, MO, United States) with a flow rate of 1 ml/min. The fluorescence detector was set at excitation wavelength of 250 nm and emission wavelength of 395 nm.
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