S1P was purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). FTY720 (2-amino-2-[2-(4-octyl-phenyl)-ethyl]-propane-1,3-diol hydrochloride), fMLP, lipopolysaccharides (LPSs) and ethylenediaminetetraacetic were obtained from Sigma-Aldrich (St. Louis, MO, USA). 2′,7′-dichloroflourescin diacetate (H2DCFDA) was purchased from ThermoFisher Scientific (Waltham, MA, USA).
Lipopolysaccharides lpss
Manufactured by Merck Group
Sourced in United States
Lipopolysaccharides (LPSs) are naturally occurring, complex molecules found in the outer membrane of Gram-negative bacteria. LPSs are essential structural components of the bacterial cell wall and play a crucial role in the bacteria's interaction with their environment.
Automatically generated - may contain errors
Lab products found in correlation
Fty720, by Merck Group (1 mentions)
Sphingosine 1 phosphate (s1p), by Enzo Life Sciences (1 mentions)
Formyl methionyl leucyl phenylalanine (fmlp), by Merck Group (1 mentions)
Penicillin streptomycin, by Procell (1 mentions)
Fetal bovine serum (fbs), by Procell (1 mentions)
Phosphate buffered saline (pbs), by Procell (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
Ripa buffer, by Solarbio (1 mentions)
Il 1β, by Bioswamp (1 mentions)
Tnf α, by Bioswamp (1 mentions)
Interleukin 6 (il 6), by Bioswamp (1 mentions)
Trypsin, by Procell (1 mentions)
Anti iκbα, by Santa Cruz Biotechnology (1 mentions)
Mouse tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Anti glyceraldehyde 3 phosphate dehydrogenase, by Santa Cruz Biotechnology (1 mentions)
3 protocols using lipopolysaccharides lpss
1
Quantifying Cellular Oxidative Stress
2
Kaempferol Glycoside Modulates Lipid Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MH-S and HepG2 cells were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China); kaempferol 3-O-α-L-rhamnoside-7-O-β-D-xylosyl(1→2)-O-α-L-rhamnoside (PubChem CID: 74978103, SGPF) was obtained from the TCM and Ethnomedicine Innovation & Development International Laboratory Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine (Changsha, China), the extract was identified by Prof. Wei Wang, and a specimen (no. 20200911) was deposited in the lab; RPMI1640 Medium (RP-1640), MEM (obtaining NEAA), fetal bovine serum (FBS), trypsin, penicillin/streptomycin, and PBS were obtained from Procell (Wuhan, China); lipopolysaccharides (LPSs) were obtained from Sigma (St. Louis, MO, USA); oil acid (OA) was obtained from Macklin Co. Ltd. (Shanghai, China); CCK-8 was purchased from Seven Biotech (Beijing, China); RIPA buffer and PMSF were purchased from Solarbio (Beijing, China); TNF-α, IL-1β, IL-6, and IL-10 ELISA kits were purchased from Bioswamp (Wuhan, China); and a tissue total cholesterol assay kit, tissue total triglyceride assay kit, liquid sample MDA assay kit, and tissue SOD assay kit were purchased from Applygen Technologies Co., Ltd. (Beijing, China).
3
Lipopolysaccharide-Induced Inflammatory Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lipopolysaccharides (LPSs) were purchased from Sigma-Aldrich (St Louis, MO, USA). In addition, eBioscience (San Diego, CA, USA) was the source of the mouse IL-6 enzyme-linked immunosorbent assay (ELISA) kit and mouse TNF-α ELISA kit. Anti-glyceraldehyde 3-phosphate dehydrogenase, anti-IκBα (nuclear factor of kappa light-polypeptide gene enhancer in B-cells inhibitor alpha), and anti-extracellular signal-regulated kinase (ERK) antibodies were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!