Columbia blood agar (COS) (bioMérieux) were used to culture the isolates. Cation-adjusted Mueller-Hinton broth (CA-MHB) (Becton, Dickinson) was used to perform the time-kill curves, and Mueller-Hinton agar (MH) plates (bioMérieux) were used for the quantification of CFU per milliliter.
The antibiotics used were meropenem, aztreonam, colistin, and amikacin (all from Sigma-Aldrich), and ceftolozane-tazobactam (Zerbaxa; MSD). The antibiotic stocks were prepared according to the Clinical and Laboratory Standards Institute (CLSI). The final concentrations used in the time-kill assays corresponded to the AUC over 24 h (Table 1 ) (17 (link)– (link)21 (link)).
The antibiotics used were meropenem, aztreonam, colistin, and amikacin (all from Sigma-Aldrich), and ceftolozane-tazobactam (Zerbaxa; MSD). The antibiotic stocks were prepared according to the Clinical and Laboratory Standards Institute (CLSI). The final concentrations used in the time-kill assays corresponded to the AUC over 24 h (