All animal experiments obtained approval from the Animal Care Committee of PLA General Hospital of Southern Theatre Command, Guangzhou, China. PKH26-labeled hASCs were seeded on 3D-printed β-TCP scaffolds and treated with or without 0.05 µg/mL NGR1 for 3 h. Male nude mice (6 weeks old, 18–20 mg bodyweight) were anesthetized by intraperitoneal injection of 1% pentobarbital. Cell-scaffold constructs were carefully implanted in each of the two dorsal subcutaneous pockets. After implantation, the skin was closed using black non-resorbable 5-0 Mersilk sutures (Ethicon, Shanghai, China). Fluorescent images were acquired by the Living Image software with the IVIS Lumina LT Series III system (PerkinElmer, Hopkinton, MA, USA). The autofluorescence background signal intensity was subtracted. The fluorescence intensity was represented by a multicolor scale ranging from red (least intense) to yellow (most intense). Signal intensity images were superimposed over grayscale reference photographs for anatomical representations. In all experiments, signals were collected from a defined ROI using the contour ROI tool, and total radiant efficiency ([p/s]/[μM/cm2]) was analyzed using the Living Image software, 4.5.
Ivis lumina lt series 3 system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina LT Series III system is a bioluminescence and fluorescence imaging platform designed for in vivo and in vitro research applications. It features a sensitive charge-coupled device (CCD) camera and a range of imaging capabilities for small animal and cell-based studies.
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Lab products found in correlation
7 protocols using ivis lumina lt series 3 system
1
Stem Cell-Scaffold Implantation for Tissue Regeneration
2
Metastatic Oral Cancer Model in Nude Mice
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Oral cancer SAS cell lines (sh-Ctrl#1 and sh-HIFCAR#6) used for metastasis model were all labelled with firefly luciferase as described above. In brief, 5 × 105 SAS cells (sh-Ctrl#1 and sh-HIFCAR#6, respectively) suspended in 0.5 ml of Matrigel (BD Bioscience) were injected into the tail veins of 6- to 8-week-old female athymic nude mice (nu/nu). Subsequently, the mice were monitored for metastases using the IVIS Lumina LT series III system (PerkinElmer) after intraperitoneal injection of luciferin once a week for 6 weeks after tail vein xenografting. Then, the lungs of the nude mice were excised post mortem for histology examination and haematoxylin–eosin staining. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment. The animal studies were approved by National Health Research Institutes Institutional Animal Care and Use Committee (approval number: NHRI-IACUC-102078) and carried out under the institutional guidelines with animal welfare standards.
3
In Vivo Bioluminescence Imaging
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Bioluminescence imaging of live animals was performed with an IVIS Lumina LT series III system (Perkin Elmer, Waltham, MA) as previously described (16 (link)). Infected mice were anesthetized with isoflurane, and Xenolight d -luciferin substrate (Perkin Elmer) was injected i.p. (150 μg/g body weight) 8 to 10 min prior to live imaging. Animals remained under isoflurane sedation for the duration of each imaging session, which occurred at various times postinfection until death or total virus clearance was achieved. Luminescent exposures were collected for 1 to 60 s with small or medium binning factors and various f-stop settings. Living Image Software (Perkin Elmer) was used for acquisition and analysis. For photon flux, a single region of interest was drawn around the entire body, and light emission was measured in photons per second per square centimeter per steradian.
4
In Vivo Bioluminescence Imaging
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Bioluminescence imaging of live animals was performed with an IVIS Lumina LT Series III system (Perkin Elmer, Waltham, MA). Infected mice were anesthetized with isoflurane and Xenolight D-luciferin substrate (Perkin Elmer, Waltham, MA) was injected intraperitoneally (150 μg/g body weight) 10 min prior to live imaging. Animals remained under isoflurane sedation for the duration of each imaging session which occurred at various timepoints post-infection until death or total viral clearance was achieved. Luminescent exposures were collected for 1–60 s with small or medium binning factors and various f-stop settings. Living Image Software (Perkin Elmer) was used for acquisition and analysis. For photon flux, a single region of interest was drawn around the entire body and light emission was measured in photons/sec/cd2/sr.
5
IN and IM Vaccination of Mice
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For IN vaccination of mice, 50 µL of 4 × 108 PFU/mL was delivered into the right nostril. For IM vaccination, 50 µL of 2 × 108 PFU/mL was injected into each hind leg of the mouse. To monitor vaccine virus infection in-vivo, an IVIS Lumina LT Series III System (Perkin-Elmer) was utilized. Mice were sedated and D-luciferin substrate was injected intraperitoneally. Luminescence was detected with an IVIS imager using the same exposure, binning and f-stop settings for each mouse.
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Bioluminescence Imaging of Antares2-MVs
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Antares2–MVs were prepared from the culture of engineered S. aureus USA300/ Eno–Antares2, as described above. For in vitro bioluminescence imaging, 50 μL of Antares2–MVs were diluted to various concentrations (2.5, 0.5, 0.1, 0.02, 0.004, 0.0008, and 0 μg/100 μL) in a black 96-well plate, and normal MVs (2.5 μg) were used as controls. The substrate hydrofurimazine (HFZ) of luciferase Antares2 was dissolved in a polyethylene glycol-300 (PEG-300) formulation containing 10% (v/v) glycerol, 10% ethanol (v/v), 10% (v/v) hydroxypropylcyclodextrin, and 35% (w/v) PEG-300.27 (link) Equal volumes of the HFZ substrate (100 μM) were subsequently added and mixed well. The total photon flux (p/sec/cm²/sr, p/s) in each well was determined using an IVIS® Lumina LT Series III system (PerkinElmer, USA).
7
Tumor growth monitoring with Antares2-MVs
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C57BL/6 and BALB/c female mice were subcutaneously inoculated with B16F10 and CT26 tumor cells (2.5 × 106 cells per mouse). A total of 5 μg of Antares2–MVs were intravenously administered to each mouse (n = 3 per group). Injection of 100 μL of HFZ substrate (100 μM) around the tumor was performed 1, 3, 6, 12, and 24 h post-injection of MVs. Total photon flux and images were obtained using an IVIS® Lumina LT Series III system (Perkin Elmer, USA).
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