C18 0

Manufactured by Merck Group

C18:0 is a type of fatty acid that is commonly used in laboratory equipment. It serves as a core component in various analytical and purification processes. The detailed technical specifications and intended applications of this product are not readily available from our marketing materials.

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Lab products found in correlation

C16 0, by Merck Group (3 mentions) Apexcontrol 3, by Bruker (2 mentions) Esi tuning mix part no g2432a, by Agilent Technologies (2 mentions) Boron trifluoride, by Merck Group (1 mentions) Fatty acid standard, by Merck Group (1 mentions) C16 1, by Merck Group (1 mentions) C17 0, by Merck Group (1 mentions) 9.4 t apex ultra hybrid qh fticr ms, by Bruker (1 mentions)

3 protocols using c18 0

Fatty Acid Determination Protocol

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Chloroform, ethyl alcohol (96% w/w), methanol, n-hexane, formic acid (99% w/w) hydrochloric acid (37% w/w) and anhydrous sodium sulphate were of analytical grade and were purchased from Carlo Erba (Milan, Italy). Boron trifluoride (approximately 10% w/w in methanol for gas chromatography (GC) derivatization) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Fatty acid standards (C16:0, C16:1, C17:0, C18:0, C18:1, C18:2, C18:3) were also purchased from Sigma-Aldrich.

Narducci V., Finotti E., Galli V, & Carcea M. (2019). Lipids and Fatty Acids in Italian Durum Wheat (Triticum durum Desf.) Cultivars. Foods, 8(6), 223.

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Lipid Profiling Using FTICR-MS

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Lipid profiling was performed using a 9.4 T Apex-ultra^TM hybrid Qh-FTICR mass spectrometer (Bruker Daltonics, Billerica, MA, USA) equipped with a 200 Hz, 355 nm Nd:YAG laser. In positive ion mode, Instrument calibration was performed using a phospholipid mixture (PC 24:0 at m/z 622.44423, PC 32:0 at m/z 734.56943, PC 36:0 at m/z 790.63203, and PC 44:2 at m/z 898.72593 from Avanti Polar Lipids, Inc.), and mass spectra were obtained over the m/z range of 500 ~1000. Three commercially available standard FAs (C_16:0 at m/z 255.23296, C_18:0 at m/z 283.26425, and C_22:6 at m/z 327.23294 from Sigma-Aldrich) with the ESI Tuning Mix (Part No. G2432A, Agilent Technology, Inc.) were used for instrument calibration over the m/z range of 100~1000 in negative ion mode. For data acquisition, a mass spectrum was accumulated in broadband mode by three full scans once with 100 laser shots using 1Mb data points over the above-mentioned ranges in the positive and negative ion modes, respectively. Ten mass spectra were randomly collected over a whole slide. All spectra were acquired using ApexControl 3.0.0 (Bruker Daltonics).

He M., Guo S., Ren J, & Li Z. (2016). In Situ Characterizing Membrane Lipid Phenotype of Human Lung Cancer Cell Lines Using Mass Spectrometry Profiling. Journal of Cancer, 7(7), 810-816.

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Hybrid FTICR-MS Lipid Profiling

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All experiments were performed using a 9.4 T Apex-ultra^TM hybrid Qh-FTICR MS (Bruker Daltonics, Billerica, MA, USA) equipped with a 200 Hz, 355 nm Nd: YAG laser. All spectra were acquired using ApexControl 3.0.0 (Bruker Daltonics). A lipid mixture (PC(24:0) at m/z 622.44423, PC(32:0) at m/z 734.56943, PC(36:0) at m/z 790.63203, and PC(44:2) at m/z 898.72593 from Avanti Polar Lipids, Inc.) was used to calibrate the instrument over the m/z range of 600 ~ 1000 in the positive ion mode and a FA mixture of three commercially-available standard (C_16:0 at m/z 255.23296, C_18:0 at m/z 283.26425, and C_22:0 at m/z 339.32684 from Sigma-Aldrich) combined with the ESI Tuning Mix (Part No. G2432A, Agilent Technology, Inc.) was used to calibrate the instrument over the m/z range of 100 ~ 1000 in the negative ion mode. For membrane lipid profiling, a mass spectrum was accumulated with three full scans once with 100 laser shots in the positive and negative ion modes, respectively. For each slide, eight mass spectra were randomly collected.

He M., Guo S, & Li Z. (2015). In situ characterizing membrane lipid phenotype of breast cancer cells using mass spectrometry profiling. Scientific Reports, 5, 11298.

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