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Membrane integrity wb antibody cocktail

Manufactured by Abcam
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Sourced in Japan

The Membrane Integrity WB Antibody Cocktail is a reagent designed for the detection of membrane integrity in Western blotting applications. It contains a combination of antibodies that target specific proteins associated with cell membrane integrity.

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Lab products found in correlation

Anti v5 antibody, by Thermo Fisher Scientific (2 mentions) Anti actin, by Merck Group (2 mentions) Sqstm1, by BD (1 mentions) Horseradish peroxidase linked secondary antibody, by Cell Signaling Technology (1 mentions) P crkl, by Cell Signaling Technology (1 mentions) Tubb β tubulin, by Cell Signaling Technology (1 mentions)

3 protocols using membrane integrity wb antibody cocktail

1

Western Blotting of Autophagy Signaling

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Western blotting was performed using antibodies against p-CRKL (Cell Signaling Technology, 3181), LC3B (Cell Signaling Technology, 2775) TUBB/β-tubulin (Cell Signaling Technology, 2146), SQSTM1 (BD Biosciences, 610833), ATG7 (Epitomics, 2054–1) and Membrane Integrity WB Antibody Cocktail (Abcam, ab110414). Primary antibody detection was by enhanced chemiluminescence (GE Healthcare/Amersham, RPN2106) using a horseradish peroxidase-linked secondary antibody (Cell Signaling Technology, 7074 and 7076).
Karvela M., Baquero P., Kuntz E.M., Mukhopadhyay A., Mitchell R., Allan E.K., Chan E., Kranc K.R., Calabretta B., Salomoni P., Gottlieb E., Holyoake T.L, & Helgason G.V. (2016). ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells. Autophagy, 12(6), 936-948.
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2

Detailed Antibody Characterization Protocol

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The antibodies used in this study were: anti-V5 antibody purchased from Thermo Fisher Scientific (Waltham, MA), anti-TOM20 (612278) from BD Transduction Laboratories (San Jose, CA), anti-MFN2 (D2D10) and –SDHA (D6J9M) antibodies from Cell Signaling Technology (Danvers, MA), anti-GFP antibody (GF200) from Nacalai Tesque (Kyoto, Japan), Membrane Integrity WB Antibody Cocktail (anti-CVa, -Core1, -porin, -CypD, and -CytC antibodies) and anti-MTCO1 antibody (1D6E1A8) from Abcam (Cambridge, UK), anti-myc antibody (M192–3) from MBL (Nagoya, Japan), anti-actin from Sigma-Aldrich (St. Louis, MO), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (5A12) from Wako Pure Chemicals (Osaka, Japan). Anti-StarD7 antibody was prepared as reported previously14 (link).
Horibata Y., Ando H., Satou M., Shimizu H., Mitsuhashi S., Shimizu Y., Itoh M, & Sugimoto H. (2017). Identification of the N-terminal transmembrane domain of StarD7 and its importance for mitochondrial outer membrane localization and phosphatidylcholine transfer. Scientific Reports, 7, 8793.
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3

Antibody Inventory for Protein Detection

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The antibodies used in this study were: anti-myc antibody (M192-3) from MBL (Nagoya, Japan), anti-SDHA (D6J9M) and anti-tubulin β (9F3#5346) antibodies from Cell Signaling Technology (Danvers, MA), anti-YME1L1 (11510-1-AP) and anti-MYH4 (20140-1-AP) antibodies from Proteintech (Chicago, IL), anti-TOM70 antibody (sc-390545) from Santa Cruz Biotechnology, Inc. (Dallas, TX), Membrane Integrity WB Antibody Cocktail (anti-CVa, -Core1, -porin, -CypD, and -CytC antibodies) and anti-PARL antibody (ab45231) from Abcam (Cambridge, UK), anti-MYH6 (NB300-284) and anti-myogenin (NBP2-32986) antibodies from Norus Biologicals (Littleton, CO), anti-actin from Sigma-Aldrich (St. Louis, MO), anti-V5 antibody from Thermo Fisher Scientific (Waltham, MA), and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (5A12) from Wako Pure Chemicals (Osaka, Japan). Anti-MyoD antibody (Ab-200) was from GenScript (Piscataway, NJ). Anti-StarD7 antibody was prepared as reported previously14 (link).
Horibata Y., Mitsuhashi S., Shimizu H., Maejima S., Sakamoto H., Aoyama C., Ando H, & Sugimoto H. (2020). The phosphatidylcholine transfer protein StarD7 is important for myogenic differentiation in mouse myoblast C2C12 cells and human primary skeletal myoblasts. Scientific Reports, 10, 2845.
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