Log In Sign up
The largest database of trusted experimental protocols

Mbptrap hd 5ml columns

Manufactured by GE Healthcare
Visit Supplier

The MBPTrap HD 5mL columns are designed for the purification of histidine-tagged proteins. The columns feature a high-capacity matrix that is optimized for fast and efficient protein capture and elution.

Automatically generated - may contain errors

Lab products found in correlation

Fm4 64, by Thermo Fisher Scientific (6 mentions) Dextrin sepharose high performance, by GE Healthcare (2 mentions) Ni nta agarose, by Thermo Fisher Scientific (2 mentions) Peroxidase conjugated secondary antibody, by Bio-Rad (2 mentions) D1817, by Thermo Fisher Scientific (2 mentions) Alexa 647 egf, by Thermo Fisher Scientific (2 mentions) Alexa555 egf, by Thermo Fisher Scientific (2 mentions) Tritc dextran, by Thermo Fisher Scientific (2 mentions) L7528, by Thermo Fisher Scientific (2 mentions) E35351, by Thermo Fisher Scientific (2 mentions) E35350, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 tfp ester, by Thermo Fisher Scientific (2 mentions) L leucyl l leucine methyl ester llome, by Merck Group (2 mentions) Ez link sulfo nhs ss biotin, by Thermo Fisher Scientific (2 mentions) Lysotracker, by Thermo Fisher Scientific (2 mentions) Glutathione sepharose 4b, by GE Healthcare (2 mentions) Dspe peg biotin, by Avanti Polar Lipids (2 mentions) Lissamine rhodamine b sulfonyl 18 1 rhod pe, by Avanti Polar Lipids (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions)

2 protocols using mbptrap hd 5ml columns

1

Fluorescent Probes for Cell Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
We obtained FM4-64, LysoTracker, Alexa 647-EGF, Alexa-555 EGF and TRITC-dextran (T13320, L7528, E35351, E35350 and D1817, respectively) from Thermo Fisher Scientific (Waltham, MA), as well as EZ-Link Sulfo-NHS-SS-Biotin, Alexa Fluor 488 TFP ester, Ni-NTA Agarose, 7 kDa MWCO Zeba Spin Desalting Columns and Hoechst 33342. Peroxidase-conjugated secondary antibodies were from Bio-Rad Laboratories (Hercules, CA), MBPTrap HD 5mL columns, Glutathione Sepharose 4B and Dextrin Sepharose High Performance from GE Healthcare (Anaheim, CA), and the cOmplete Protease Inhibitor Cocktail from Roche (Basel, Switzerland). We obtained DOPC, DSPE-PEG-Biotin, DOPE, DOPS and lissamine rhodamine B sulfonyl (18:1) Rhod PE from Avanti Polar Lipids (Alabaster, AL, and the lysosomotropic reagent L-leucyl-L-leucine methyl ester (LLOMe) from Sigma-Aldrich (St. Louis, MO). Other reagents and chemicals were obtained from Sigma-Aldrich (St. Louis, MO)
Mercier V., Larios J., Molinard G., Goujon A., Matile S., Gruenberg J, & Roux A. (2020). Endosomal Membrane Tension Regulates Escrt-III-Dependent Intra-Lumenal Vesicle Formation. Nature cell biology, 22(8), 947-959.
+ Open protocol
+ Expand
2

Fluorescent Probes for Cell Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
We obtained FM4-64, LysoTracker, Alexa 647-EGF, Alexa-555 EGF and TRITC-dextran (T13320, L7528, E35351, E35350 and D1817, respectively) from Thermo Fisher Scientific (Waltham, MA), as well as EZ-Link Sulfo-NHS-SS-Biotin, Alexa Fluor 488 TFP ester, Ni-NTA Agarose, 7 kDa MWCO Zeba Spin Desalting Columns and Hoechst 33342. Peroxidase-conjugated secondary antibodies were from Bio-Rad Laboratories (Hercules, CA), MBPTrap HD 5mL columns, Glutathione Sepharose 4B and Dextrin Sepharose High Performance from GE Healthcare (Anaheim, CA), and the cOmplete Protease Inhibitor Cocktail from Roche (Basel, Switzerland). We obtained DOPC, DSPE-PEG-Biotin, DOPE, DOPS and lissamine rhodamine B sulfonyl (18:1) Rhod PE from Avanti Polar Lipids (Alabaster, AL, and the lysosomotropic reagent L-leucyl-L-leucine methyl ester (LLOMe) from Sigma-Aldrich (St. Louis, MO). Other reagents and chemicals were obtained from Sigma-Aldrich (St. Louis, MO)
Mercier V., Larios J., Molinard G., Goujon A., Matile S., Gruenberg J, & Roux A. (2020). Endosomal Membrane Tension Regulates Escrt-III-Dependent Intra-Lumenal Vesicle Formation. Nature cell biology, 22(8), 947-959.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!