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Lag 3

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LAG-3 is a cell surface protein that functions as an immune checkpoint molecule. It is expressed on activated T cells and plays a role in regulating T cell activity and immune response.

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Lab products found in correlation

Annexin 5, by Santa Cruz Biotechnology (2 mentions) Lag 3, by BD (2 mentions) Cd62l, by BD (2 mentions) Bcl 2 bcl 10c4, by BioLegend (2 mentions) Cd101, by BioLegend (2 mentions) 7 amino actinomycin, by BD (2 mentions) Bcl xl h 5, by Santa Cruz Biotechnology (2 mentions) Ctla 4, by Cytek Biosciences (2 mentions) Aqua live dead dye, by Thermo Fisher Scientific (1 mentions) Foxp3 fitc, by Thermo Fisher Scientific (1 mentions) Cd8 percp, by BioLegend (1 mentions) Cd39 bv421, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Ki67 alexafluor700, by BD (1 mentions) Cd3 pecy5, by Thermo Fisher Scientific (1 mentions) Tim 3 pe cy7, by BioLegend (1 mentions) Btla pe, by BioLegend (1 mentions) Cd25 bv605, by BioLegend (1 mentions) Ctla 4 pe cf594, by BD (1 mentions) 7 aad, by BioLegend (1 mentions)

Close spelling variants of this product

LAG-3–FITC (2 citations) LAG-3 (17B4, (2 citations)

4 protocols using lag 3

1

Comprehensive Antibody Panel for Mouse and Human T Cell Phenotyping

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For mouse studies, the following antibodies were purchased: from BioLegend: 2B4 (m2B4), BCL-2 (BCL/10C4), CD101 (Moushi101), CD11c (N418), CD127 (A7R34), CD19 (6D5), CD25 (PC61.5), CD3 (145–2C11), CD38 (90), CD39 (Duha59), CD40 (3/23), CD44 (IM7), CD62L (MEL-14), CD69 (H1.2F3), CD70 (FR70), CD80 (16–10A1), CD86 (GL-1), CD90.1 (OX-7 and HIS51), CD90.2 (30-H12 and 53–2.1), CXCR5 (L138D7), Eomes (Dan11mag), GZMB (GB11), IFNγ (XMG1.2), IL-2 (JES6–5H4), KLRG1 (2F1), LAG-3 (C9B7W), MHC I-A/I-E (M5/114.15.2), PD-1 (RMP1–30), T-bet (4B10), TIM-3 (RMT3–23), TNF (MP6-XT22), and 7-amino-actinomycin (7-AAD); from BD Biosciences: annexin V, CD95 (Jo2), Ki67 (B56), Vb7 (TR310); BCL-xL (H-5; Santa Cruz Biotechnology); BIM (C34C5; Cell Signaling Technology), CD8 (53–6.7; eBioscience), CTLA-4 (UC10–410-11; Tonbo Biosciences), TCF-1 (C63D9; Cell Signaling Technology), TIGIT (GIGD7; eBioscience).
For human studies, the following antibodies were purchased: CD39 (A1; BioLegend), CD45RA (HI100; BioLegend), CD45RO (UCHL1; BioLegend), CD8 (RPA-T8; BioLegend), LAG-3 (17B4; Enzo Life Sciences), PD-1 (EH12.1; BD Biosciences) and TIM-3 (F38–2E2; BioLegend).
For flow cytometric detection and analysis of mouse and human TOX, anti-human/mouse TOX antibody clone REA473 was used (Miltenyi Biotec); antibody clone REA293 was used as TOX isotype (Miltenyi Biotec).
Scott A.C., Dündar F., Zumbo P., Chandran S.S., Klebanoff C.A., Shakiba M., Trivedi P., Menocal L., Appleby H., Camara S., Zamarin D., Walther T., Snyder A., Femia M.R., Comen E.A., Wen H.Y., Hellmann M.D., Anandasabapathy N., Liu Y., Altorki N.K., Lauer P., Levy O., Glickman M.S., Kaye J., Betel D., Philip M, & Schietinger A. (2019). TOX is a critical regulator of tumour-specific T cell differentiation. Nature, 571(7764), 270-274.
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2

Comprehensive Antibody Panel for Mouse and Human T Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
For mouse studies, the following antibodies were purchased: from BioLegend: 2B4 (m2B4), BCL-2 (BCL/10C4), CD101 (Moushi101), CD11c (N418), CD127 (A7R34), CD19 (6D5), CD25 (PC61.5), CD3 (145–2C11), CD38 (90), CD39 (Duha59), CD40 (3/23), CD44 (IM7), CD62L (MEL-14), CD69 (H1.2F3), CD70 (FR70), CD80 (16–10A1), CD86 (GL-1), CD90.1 (OX-7 and HIS51), CD90.2 (30-H12 and 53–2.1), CXCR5 (L138D7), Eomes (Dan11mag), GZMB (GB11), IFNγ (XMG1.2), IL-2 (JES6–5H4), KLRG1 (2F1), LAG-3 (C9B7W), MHC I-A/I-E (M5/114.15.2), PD-1 (RMP1–30), T-bet (4B10), TIM-3 (RMT3–23), TNF (MP6-XT22), and 7-amino-actinomycin (7-AAD); from BD Biosciences: annexin V, CD95 (Jo2), Ki67 (B56), Vb7 (TR310); BCL-xL (H-5; Santa Cruz Biotechnology); BIM (C34C5; Cell Signaling Technology), CD8 (53–6.7; eBioscience), CTLA-4 (UC10–410-11; Tonbo Biosciences), TCF-1 (C63D9; Cell Signaling Technology), TIGIT (GIGD7; eBioscience).
For human studies, the following antibodies were purchased: CD39 (A1; BioLegend), CD45RA (HI100; BioLegend), CD45RO (UCHL1; BioLegend), CD8 (RPA-T8; BioLegend), LAG-3 (17B4; Enzo Life Sciences), PD-1 (EH12.1; BD Biosciences) and TIM-3 (F38–2E2; BioLegend).
For flow cytometric detection and analysis of mouse and human TOX, anti-human/mouse TOX antibody clone REA473 was used (Miltenyi Biotec); antibody clone REA293 was used as TOX isotype (Miltenyi Biotec).
Scott A.C., Dündar F., Zumbo P., Chandran S.S., Klebanoff C.A., Shakiba M., Trivedi P., Menocal L., Appleby H., Camara S., Zamarin D., Walther T., Snyder A., Femia M.R., Comen E.A., Wen H.Y., Hellmann M.D., Anandasabapathy N., Liu Y., Altorki N.K., Lauer P., Levy O., Glickman M.S., Kaye J., Betel D., Philip M, & Schietinger A. (2019). TOX is a critical regulator of tumour-specific T cell differentiation. Nature, 571(7764), 270-274.
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3

Comprehensive Immune Cell Profiling

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GICs and PBMCs were stained for CD3-PE-Cy5.5 (eBioscience), CD4-BV711, CD8-PerCP, CD25-BV605, CD45RA-BV570, CCR7-BV650, CD39-BV421, PD-1-APC-Cy, BTLA-PE, Tim-3-PE-Cy7 (all Biolegend), LAG3 (Enzo Life Sciences, Lörrach, Germany), CTLA4-PE-CF594 (BD) and Live/dead-Aqua dye (Life technologies, Carlsbad, CA) or with isotype controls. Cells were fixed and permeabilized, followed by ICS using Foxp3-FITC (eBioscience) and Ki67-Alexa Fluor 700 (BD) and measured on an LSR Fortessa (BD).
Rammensee H.G., Wiesmüller K.H., Chandran P.A., Zelba H., Rusch E., Gouttefangeas C., Kowalewski D.J., Di Marco M., Haen S.P., Walz J.S., Gloria Y.C., Bödder J., Schertel J.M., Tunger A., Müller L., Kießler M., Wehner R., Schmitz M., Jakobi M., Schneiderhan-Marra N., Klein R., Laske K., Artzner K., Backert L., Schuster H., Schwenck J., Weber A.N., Pichler B.J., Kneilling M., la Fougère C., Forchhammer S., Metzler G., Bauer J., Weide B., Schippert W., Stevanović S, & Löffler M.W. (2019). A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer. Journal for Immunotherapy of Cancer, 7, 307.
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4

Comprehensive Immune Cell Profiling

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Cellular composition of leukapheresis samples was determined by flow cytometry using the following antibodies (20 min at 4°C): CD3 (BD), CD19 (BioLegend), and CD56 (BioLegend).
Expression of exhaustion markers was determined by flow cytometry using the following antibodies (20 min at 4°C): CD3 (BD), RQR8 (Qbend10 antibody; R&D), CD8 (BioLegend), LAG3 (Enzo Life Sciences), PD1 (BioLegend), and TIM3 (BioLegend).
All samples were counterstained with 7AAD (BioLegend) to exclude dead cells.
BD Celesta was used for cell acquisition, and data were analyzed using FlowJo V10 (Treestar). Representative plots showing the gating strategy are shown in Supplementary Figure 6A.
Wang X., Peticone C., Kotsopoulou E., Göttgens B, & Calero-Nieto F.J. (2021). Single-cell transcriptome analysis of CAR T-cell products reveals subpopulations, stimulation, and exhaustion signatures. Oncoimmunology, 10(1), 1866287.
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