1H NMR spectra were obtained on a Bruker Fourier transform spectrometer (500 MHz) at 25 oC. 13C NMR spectra were recorded on a Bruker Fourier transform spectrometer (125 MHz) spectrometer. All NMR spectra were calibrated using the residual solvent (CDCl3) as internal reference (1H NMR = 7.26, 13C NMR = 77.16). All chemical shifts were reported in parts per million (ppm) and coupling constants (J) in Hz. The following abbreviations were used to explain the multiplicities: d = doublet, t = triplet, m = multiplet. IR spectra were taken on a Bruker Vector 22 spectrophotometer as KBr pellets. High resolution mass spectra (HRMS) were measured on an Agilent 6224 TOF LC/MS spectrometer using ESI-TOF (electrospray ionization-time of flight). The reaction process between AP and H2O2 was monitored on a SHIMADZU LCMS-2020 spectrometer. UV-Vis spectra were taken on a HITACHI U-3010 Spectrophotometer. Fluorescence measurements were conducted on an Agilent Cary Eclipse Fluorescence Spectrophotometer with slit widths to be 10 and 10 nm for excitation and emission respectively, and the photomultiplier (PMT) detector voltage was set at medium.
6224 tof lc ms spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The 6224 TOF LC/MS spectrometer is an analytical instrument designed for high-performance liquid chromatography and time-of-flight mass spectrometry. It provides accurate mass measurement and high-resolution capabilities for a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
U 3010 spectrophotometer, by Hitachi (3 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (2 mentions)
Lcms 2020 spectrometer, by Shimadzu (1 mentions)
1100 series hplc system, by Agilent Technologies (1 mentions)
Eclipse xdb c18, by Agilent Technologies (1 mentions)
Delsa nano c particle analyzer, by Beckman Coulter (1 mentions)
Vector 22 spectrophotometer, by Bruker (1 mentions)
Ls 45 fluorescence spectrometer, by PerkinElmer (1 mentions)
5 protocols using 6224 tof lc ms spectrometer
1
Spectroscopic Characterization of AP-H2O2 Reaction
2
Synthesis and Characterization of Novel Organic Compounds
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Unless otherwise noted, all reagents were purchased from commercial suppliers and used without further purification. Anhydrous THF was distilled from Na prior to use. Reactions were monitored by thin layer chromatography using TLC Silica gel 60 F254 supplied by Qingdao Puke Separation Material Corporation (Qingdao, China). Silica gel for column chromatography was 200–300 mesh and was supplied by Qingdao Marine Chemical Factory (Qingdao, China). Characterization of intermediates and final compounds was done using NMR spectroscopy and mass spectrometry. 1H-NMR spectra (500 MHz) were determined in CDCl3 on an Advance III MHz spectrometer (Bruker, Bremen, Germany) with TMS as internal standard. Chemical shifts are expressed in parts per million (ppm) and coupling constants in Hz. Mass spectra (ESI-MS) were recorded on an Esquire-LC-00075 spectrometer (Bruker, Bremen, Germany). HRMS were recorded on a 6224 TOF LC/MS spectrometer (Agilent, Santa Clara, CA, USA). Purity was confirmed on a Agilent 1100 series HPLC system equipped with a C18 column (Eclipse XDB-C18, 5 μm, 4.6 × 250 mm) eluted in gradient mode with CH3CN in H2O (from 10% to 95%). Melting points were measured with a B-540 melting-point apparatus (Büchi, Flawil, St. Gallen, Switzerland) and are uncorrected.
3
NMR, IR, and Optical Characterization
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1H and 13C NMR spectra were recorded on a Bruker Fourier transform 400 NMR spectrometer at 25oC using CDCl3 as solvent and were calibrated referencing residual undeuterated solvent as an internal reference (1H NMR = 7.26, 13C NMR = 77.16). All chemical shifts were given in ppm and coupling constants (J) in Hz. The following abbreviations or combinations thereof were used to explain the multiplicities: s = singlet, t = triplet, m = multiplet. IR spectra were recorded on a Bruker Vector 22 spectrophotometer as KBr pellets. High resolution mass spectra (HRMS) were recorded on an Agilent 6224 TOF LC/MS spectrometer using ESI-TOF (electrospray ionization-time of flight). Absorption spectra were acquired using a Hitachi U-3010 spectrophotometer. Fluorescence measurements were carried out on a Perkin-Elmer LS 45 fluorescence spectrometer. Dynamic light scattering (Beckman-Coulter DelsaTM Nano-C particle analyzer) was used to determine the size of the aggregated particle colloids in aqueous solution.
4
Spectroscopic Analysis of Organic Compounds
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1H and 13C NMR spectra were recorded on a Bruker Fourier transform 500 NMR spectrometer at ambient temperature. CDCl3 was used as solvent except otherwise indicated and the spectra were calibrated referencing residual undeuterated solvent as an internal reference (1H NMR = 7.26, 13C NMR = 77.16). Chemical shifts were given in ppm and coupling constants (J) in Hz. Following abbreviations were used to designate multiplicities: s = singlet, d = doublet, m = multiplet. High-resolution mass data were obtained on an Agilent 6224 TOF LC/MS spectrometer using ESI-TOF (electrospray ionization-time of flight). IR spectra were obtained on a Bruker VECTOR FTIR. UV-Vis spectra were taken on a HITACHI U-3010 Spectrophotometer. Fluorescence measurements were performed on an Agilent Cary Eclipse Fluorescence Spectrophotometer with slit widths to be 10 and 10 nm for excitation and emission respectively except otherwise indicated, and the photomultiplier (PMT) detector voltage was set at medium.
5
Synthesis of Functionalized Heterocyclic Compounds
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Dichloroglyoxime, sodium azide, triphenylphosphine, isocyanates, anhydrous sodium sulfate and the common solvents are commercially available analytical purity and can be used directly without purification. Toluene (C6H5CH3) and chloroform (CHCl3) were dried with anhydrous calcium chloride for one week.
All melting points were determined on an X-4 model melting point apparatus and uncorrected previously. IR spectra were recorded on a Perkin Elmer-Spectrum One spectrometer as KBr pellets and reported in cm-1. 1H NMR and 13C NMR spectra were acquired on AVANCE NEO 500M spectrometer (500 and 125 MHz, respectively) in DMSO and resonances relative to TMS. High-resolution mass spectra (HRMS) were performed by an Agilent 6224 TOF LC/MS spectrometer. Monitoring of the reaction progress and assessment of the purity of synthesized compounds was done by TLC on Silica gel (HSGF254) plates, and Silica gel (200-300 mesh) was used for short column chromatography.
All melting points were determined on an X-4 model melting point apparatus and uncorrected previously. IR spectra were recorded on a Perkin Elmer-Spectrum One spectrometer as KBr pellets and reported in cm-1. 1H NMR and 13C NMR spectra were acquired on AVANCE NEO 500M spectrometer (500 and 125 MHz, respectively) in DMSO and resonances relative to TMS. High-resolution mass spectra (HRMS) were performed by an Agilent 6224 TOF LC/MS spectrometer. Monitoring of the reaction progress and assessment of the purity of synthesized compounds was done by TLC on Silica gel (HSGF254) plates, and Silica gel (200-300 mesh) was used for short column chromatography.
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