Sialylated pentasaccharide standards LSTa, LSTb, and LSTc were purchased from Dextra Laboratories (Reading, UK), and GM1a and LSTd from Elicityl (Grenoble, France). Trifluoroacetic acid (TFA) of ReagentPlus grade was from Sigma-Aldrich (Gillingham, UK). All solvents used were of HPLC grade.
Reagentplus grade
Manufactured by Merck Group
Sourced in Canada
ReagentPlus grade is a high-purity laboratory reagent manufactured by Merck Group. It is designed to meet the stringent requirements of analytical and research applications. The product provides consistent quality and reliability to support accurate and reproducible results in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Nafion perfluorinated resin solution, by Merck Group (1 mentions)
Milli q reagent water, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
1 2 diphytanoyl sn glycero 3 phosphocholine dphpc, by Avanti Polar Lipids (1 mentions)
Milli q water, by Merck Group (1 mentions)
Trace metal grade, by Thermo Fisher Scientific (1 mentions)
Perchloric acid, by Thermo Fisher Scientific (1 mentions)
7 protocols using reagentplus grade
1
Sialylated Pentasaccharide Standards Purification
2
Graphene Nanoplatelets for Electrochemical Applications
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Graphite nanoplatelets (GNPs) (surface0modified friable nano-graphite) were obtained from Perpetuus Carbon Technologies Ltd (Swansea, UK). GNPs were synthesized by employing dielectric barrier discharge (DBD) plasma with various working gases [23 (link)]. Nafion perfluorinated resin solution (5 wt % in lower aliphatic alcohols and containing 15‒20% water) was purchased from Sigma-Aldrich (Gillingham, Dorset, UK). Standard caffeine powder (Reagent Plus grade) was purchased from Sigma-Aldrich. NaCl (99.5%) and ethanol (absolute HPLC grade) were purchased from Acros Organics (Geel, Belgium). Aqueous solutions of NaCl at pH 2 were prepared by adjusting the as-prepared solutions of NaCl with aliquots of 0.5 M HCl solutions. All aqueous solutions were prepared from doubly distilled Milli-Q reagent water (Millipore Corp., Burlington, MA, USA) with a resistivity of 18 MΩ·cm at 25 °C.
3
HPLC Analysis of Chlorophyll Derivatives
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Authentic samples of chlorophyll derivatives used for the identification of compounds in the high-performance liquid chromatography (HPLC) analysis were prepared using previously described methods [8 (link)–10 (link)], including chlorophylls a/b (Chls-a/b), pheophytins a/b (Phes-a/b), pyropheophytins a/b (pPhes-a/b), pheophorbide a (PPB-a), pyropheophorbide a (pPPB-a), 132,173-cyclopheophorbides a/b enol (cPPBs-aE/bE), and (132R)- and (132S)-hydroxychlorophyllones a ([R/S]-hCPLs-a). Because cPPB-aE is stabilized in deoxygenated anisole [8 (link)], all authentic standard solutions used in this study were prepared in anisole (ReagentPlus® grade, Sigma-Aldrich, St. Louis, USA). Molar concentrations were spectrographically determined with reference to the previously reported molar extinction coefficients of the Chl-a derivatives [8 (link)] using a Hitachi U-3500 spectrophotometer (Hitachi, Ltd., Tokyo, Japan).
4
Microencapsulation of Phase Change Material
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PLA (Ingeo 8052D NatureWorks LLC) was used as the polymeric shell of the microPCM. PA (Acros organics) was used as the PCM core. 87–89% hydrolyzed polyvinyl alcohol (PVA, Sigma Aldrich) with an average molecular weight of 146,000–186,000 g mol−1 and sodium dodecyl sulfate (SDS, Sigma Aldrich, ReagentPlus grade) were used as emulsifiers. Dichloromethane (DCM, Caledon Laboratory Chemical), with the density of 1320 kg m−3, was the organic solvent for the preparation of the oil phase. Deionized water was the aqueous medium. All materials and chemicals were used as received.
5
Lipid-Out Approach for Membrane Reconstitution
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We used the lipid-out approach by adding phospholipids to the interface from the oil phase. 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC, Avanti Polar Lipids, Alabaster, AL, USA) was purchased as a solution in chloroform. We transferred the appropriate amount of lipid solution into a pear-shaped flask and removed the solvent with a rotary evaporator (Büchi Labortechnik AG, Flawil, Switzerland). The remaining lipid film was dissolved in hexadecane (Reagent plus grade, Sigma-Aldrich) and squalane (Sigma-Aldrich) (1:1 (v/v)), if not stated otherwise, while being agitated by an ultrasonication bath at 50 °C for ~30 min. The final phospholipid concentration in oil was 0.5–5 mg/mL. Before use, the oil solution with phospholipids was filtrated (0.45 μm pore size, RC 4 Male Luer Slip Minisart filters, Huberlab, Switzerland).
6
Validating Collagen Concentration via UTE MRI
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Example 1
In order to validate Eq. 1 in a controlled environment, collagen solutions of varying concentrations were prepared and analyzed with UTE MRI. The collagen solutions were prepared by dissolving hydrolyzed type I and III collagen powder (NeoCell Super Collagen Type I and III Powder, NeoCell, Irvine, Calif., USA) in 0.125 mM manganese chloride (MnCl2) solvent. Types I and III were employed because they are the most abundant collagen types in the heart [20]. Pharmaceutical food grade bovine hide collagen was the source of the powder. The manganese chloride solution was prepared by dissolving the appropriate amount of manganese(II) chloride tetrahydrate (MnCl2.4H2O) (ReagentPlus grade, Sigma-Aldrich, Oakville, Ontario, Canada) in Milli-Q water (EMD Millipore, Billerica, Mass., USA). This MnCl2 solution approximated the long T2* of cardiac muscle, valued at ˜35 ms [21]. Concentrations, expressed as % mass/volume (% m/v), were as follows: 0%, 2.5%, 5%, 10%, 20%, 30%, 40%, 50%. At 50% m/v concentration, i.e. with 50 g of collagen dissolved in a solution with total volume of 100 mL, the solution approached the saturation point for collagen solubility; hence, higher concentrations were not prepared. For scanning, the solutions were placed in 5-mL glass tubes.
7
Chlorella Algae Growth Optimization
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Nitric acid (Trace metal™ grade, 67˗70% Fisher Scientific, Ontario, Canada), per-chloric acid (reagent grade 67˗71% Fisher Scientific, Ontario, Canada). Standards for metal analysis (SCP Science, plasmaCAL, Quebec, Canada). HPLC grade water was prepared in the laboratory using milli-Q/Millli-Ro Milli pore system (Milford, Massachusetts, USA). Titanium dioxide (TiO 2 ) powder (reagentPlus® grade, 99-100%, Sigma-Aldrich, Ontario, Canada). Commercial fertilizer (Miracle-Gro® with 5% N and 30% P, Quebec Canada) was used to promote Chlorella algae growth.
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