Log In Sign up
The largest database of trusted experimental protocols

Anti cd15 pacific blue

Manufactured by Beckman Coulter
Visit Supplier

Anti-CD15-Pacific Blue is a fluorochrome-conjugated monoclonal antibody that binds to the CD15 antigen. CD15 is a marker for myeloid cells, granulocytes, and some monocytes. This product is designed for flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd14 pe texas red, by Beckman Coulter (2 mentions) Anti cd38 pe cy7, by Beckman Coulter (2 mentions) Anti cd45 apc, by Beckman Coulter (2 mentions) Anti cd19 pe, by Beckman Coulter (2 mentions) Anti cd33 pe cy5, by Beckman Coulter (2 mentions) Anti cd3 fitc, by Beckman Coulter (2 mentions) Versalyse solution, by Beckman Coulter (1 mentions) Anti cd56 pe, by Beckman Coulter (1 mentions) Iotest 3 fixative solution, by Beckman Coulter (1 mentions) Anti cd4 apc, by Beckman Coulter (1 mentions) Flow count fluorosphere, by Beckman Coulter (1 mentions) Canto 2 cytometer, by BD (1 mentions)

3 protocols using anti cd15 pacific blue

1

Engraftment of AML in NSG Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal experiments were performed in accordance with institutional guidelines approved by the UHN Animal Care Committee. 8 to 12-week-old female NOD/SCID/IL-2Rgc-null (NSG) mice were sublethally irradiated (225 cGy) 6–24 hours before transplantation. Mononuclear cells from AML patients were depleted of CD3+ cells by EasySep (Stem Cell Technologies) prior to intrafemoral transplantation. Mice were sacrificed 8 or 16 weeks after transplantation and human engraftment in the injected femur and non-injected bone marrow was evaluated by flow cytometry using the following human-specific antibodies (all used at 1:200, all from BD unless stated otherwise, catalogue number in parentheses): anti-CD45-APC (340943), anti-CD19-PE, anti-CD33-PE-Cy5, anti-CD3-FITC, anti-CD14-PE Texas Red (Beckman Coulter PNIM2707U), anti-CD15-Pacific Blue (642917), anti-CD38-PE-Cy7, and anti-CD34-APC-Cy7. The threshold for detection of human engraftment was 0.1% CD45+ cells. All flow cytometric analysis was performed on the LSRII (BD Biosciences). For limiting dilution assays, the frequency of repopulating cells was calculated using ELDA software49 (link).
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
+ Open protocol
+ Expand
2

Engraftment of AML in NSG Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Animal experiments were performed in accordance with institutional guidelines approved by the UHN Animal Care Committee. 8 to 12-week-old female NOD/SCID/IL-2Rgc-null (NSG) mice were sublethally irradiated (225 cGy) 6–24 hours before transplantation. Mononuclear cells from AML patients were depleted of CD3+ cells by EasySep (Stem Cell Technologies) prior to intrafemoral transplantation. Mice were sacrificed 8 or 16 weeks after transplantation and human engraftment in the injected femur and non-injected bone marrow was evaluated by flow cytometry using the following human-specific antibodies (all used at 1:200, all from BD unless stated otherwise, catalogue number in parentheses): anti-CD45-APC (340943), anti-CD19-PE, anti-CD33-PE-Cy5, anti-CD3-FITC, anti-CD14-PE Texas Red (Beckman Coulter PNIM2707U), anti-CD15-Pacific Blue (642917), anti-CD38-PE-Cy7, and anti-CD34-APC-Cy7. The threshold for detection of human engraftment was 0.1% CD45+ cells. All flow cytometric analysis was performed on the LSRII (BD Biosciences). For limiting dilution assays, the frequency of repopulating cells was calculated using ELDA software49 (link).
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
+ Open protocol
+ Expand
3

Multi-Color Flow Cytometry for Blood Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies for blood count analysis: multi-color flow cytometry was performed using Beckman Coulter’s custom design service and its dry coating technology, and custom tubes containing anti-CD16-FITC (clone 3G8), anti-CD56-PE (clone N901), anti-CD19-PE-Cy5.5 (clone J3-119), anti-CD14-PE-Cy7 (clone RMO52), anti-CD4-APC (clone 13B8.2), anti-CD8-Alexa Fluor 700 (clone B9.11), anti-CD3-APC-Alexa Fluor 750 (clone UCTH1), anti-CD15-PacificBlue (clone 80H5) and anti-CD45-KromeOrange (clone J.33) were produced.
Staining protocol: 100 μl of total heparinized blood was added to a DURAClone tube, vortexed immediately for 15 s and incubated for 15 min at room temperature in the dark. Two milliliters of red blood lysis solution (VersaLyse solution, A09777, Beckman Coulter) containing 50 μl of the fixative agent IOTest 3 Fixative Solution (A07800, Beckman Coulter) was added, inverted and incubated for 15 min in the dark. Then, 100 µl of counting beads (Flow-Count Fluorospheres, 7547053, Beckman Coulter) was added before acquisition on a Canto II cytometer (BD Biosciences).
Thibaudin M., Fumet J.D., Chibaudel B., Bennouna J., Borg C., Martin-Babau J., Cohen R., Fonck M., Taieb J., Limagne E., Blanc J., Ballot E., Hampe L., Bon M., Daumoine S., Peroz M., Mananet H., Derangère V., Boidot R., Michaud H.A., Laheurte C., Adotevi O., Bertaut A., Truntzer C, & Ghiringhelli F. (2023). First-line durvalumab and tremelimumab with chemotherapy in RAS-mutated metastatic colorectal cancer: a phase 1b/2 trial. Nature Medicine, 29(8), 2087-2098.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!