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Aqueous uranyl acetate

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2% aqueous uranyl acetate is a laboratory reagent used in the preparation of samples for transmission electron microscopy (TEM) imaging. It is a heavy metal stain that enhances the contrast of biological specimens, allowing for better visualization of cellular structures and ultrastructural details. This product is typically used in the field of materials science, life sciences, and nanotechnology research.

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Lab products found in correlation

Filter paper, by Cytiva (2 mentions) Tecnai g2 20 tem, by Thermo Fisher Scientific (2 mentions) Whatman, by Cytiva (2 mentions)

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Uranyl acetate (11 citations)

2 protocols using aqueous uranyl acetate

1

Electron Microscopy of Biomolecular Samples

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Samples were diluted to approximately 20 μg/mL and deposited on carbon type-B, 200-mesh copper grids (Ted Pella Inc.). Grids had previously been treated with an easiGlow glow discharge unit (PELCO). After a 5 min incubation at room temperature, the grids were washed with distilled water and stained with 2% aqueous uranyl acetate (BDH Chemicals) for 7 min. Excess uranyl acetate was absorbed with filter paper (Whatman), and the grids were left to dry before visualization under a Tecnai G2 20 TEM (FEI).
Winter D.L., Lebhar H., McCluskey J.B, & Glover D.J. (2023). A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates. Journal of Nanobiotechnology, 21, 66.
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2

TEM Imaging of Filament and Nanowire Samples

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Filament and nanowire samples for TEM were diluted to 40 μg mL−1 in PBS before deposition on Carbon Type-B, 200-mesh copper grids (Ted Pella Inc.), pre-treated with an easiGlow Glow Discharge Unit (PELCO). After a 5 min incubation at room temperature, the grids were washed with distilled water, and stained with 2 % aqueous uranyl acetate (BDH Chemicals) for 7 min. The stain was wicked off with filter paper (Whatman) and grids were left to dry before visualisation under a Tecnai G2 20 TEM (FEI). The digitized TEM images were analysed using ImageJ48 (link) (US National Institutes of Health) to measure filament and nanowire length.
Chen Y.X., Ing N.L., Wang F., Xu D., Sloan N.B., Lam N.T., Winter D.L., Egelman E.H., Hochbaum A.I., Clark D.S, & Glover D.J. (2020). Structural Determination of a Filamentous Chaperone to Fabricate Electronically Conductive Metalloprotein Nanowires. ACS nano, 14(6), 6559-6569.
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