Goat anti mouse igg h l secondary antibody alexa fluor 680 conjugate

Reagents were purchased or obtained from the following sources: rabbit antibody against Arg-II (#55003) was from Cell Signaling Technology (Danvers, MA, USA); mouse antibody against HIF1α (610958) was from BD Transduction Laboratories (Franklin Lakes, NJ, USA); rabbit antibody against HIF2α (PAB12124) was from Abnova (Taipei, Taiwan); rabbit antibody against podocin (PA5-37284) was from Invitrogen/Thermo Fisher Scientific (Waltham, MA, USA); mouse antibody against synaptopodin (sc-515842) and mouse antibody against ACE1 (sc-23908) were from Santa Cruz Biotechnology (Dallas, TX, USA); mouse antibody against tubulin (T5168), mouse antibody against β-actin (A5441), dimethyloxaloylglycine (DMOG), and rotenone were from Sigma-Aldrich (St. Louis, MO, USA); IRDye 800-conjugated affinity purified goat anti-rabbit IgG F(c) was purchased from LI-COR Biosciences (Lincoln, NE, USA); goat anti-mouse IgG (H + L) secondary antibody Alexa Fluor^® 680 conjugate was from Invitrogen/Thermo Fisher Scientific (Waltham, MA, USA).

Reagents were purchased or obtained from the following sources: rabbit antibody against Arg-II (#55003), cleaved-caspase 3 (#9664), and β-catenin (#8480) were from Cell Signaling Technology (Danvers, MA, USA); rabbit antibody against podocin (PA5-37284) was from Invitrogen/Thermo Fisher Scientific (Waltham, MA USA); rabbit antibody against TGF-β1 (ab215715) was from Abcam (Cambridge, United Kingdom); mouse antibody against GAPDH (10R-G109a), was from Fitzgerald (Acton, MA USA); IRDye 800-conjugated affinity purified goat anti-rabbit IgG F(c) was purchased from LI-COR Biosciences (Lincoln, NE, USA); goat anti-mouse IgG (H + L) secondary antibody Alexa Fluor^® 680 conjugate was from Invitrogen/Thermo Fisher Scientific (Waltham, MA, USA). These antibodies and their dilutions used for immunoblotting are presented in Table 1.

Reagents were purchased or obtained from the following sources: anti-myosin 1b antibody (ab194356, Abcam); anti-VCAM-1 antibody (sc-13160, Santa Cruz Technology); anti-λH2AX antibody (sc-517348, Santa Cruz Technology); ICAM-1 antibody (sc-8439, Santa Cruz Technology); anti-p21antibody (sc-6246, Santa Cruz Technology); anti-LC3A/B antibody (#4108S, Cell Signaling Technology); anti-LAMP1 antibody (#9091S, Cell Signaling Technology); anti-p62 antibody (18420-1-AP, Proteintech); anti-LRRK2 antibody (#5559S, Cell Signaling Technology); and anti-tubulin antibody (SAB4500087, Sigma-Aldrich). Duolink® In Situ Detection Reagents Red (DUO92008) was from Sigma. IRDye 800-conjugated affinity purified goat anti-rabbit IgG F(c) was purchased from LI-COR Biosciences (Lincoln, Nebraska, USA); goat anti-mouse IgG (H+L) secondary antibody Alexa Fluor® 680 conjugate, goat anti-mouse IgG (H+L) secondary antibody Alexa Fluor® 488 conjugate, goat anti-rabbit IgG (H+L) secondary Antibody Alexa Fluor® 488 conjugate, and goat anti-rabbit IgG (H+L) secondary antibody Alexa Fluor® 594 conjugate were from Invitrogen/Thermo Fisher Scientific (Waltham, MA, USA). Insulin-transferrin-selenite sodium and dexamethasone were from Sigma (St. Louis, Missouri, USA). All cell culture media and materials were purchased from Gibco/Thermo Fisher Scientific (Waltham, Massachusetts, USA).