Spss 17.0 program

Statistical analysis of obtained data was performed with the SPSS 17.0 program (SPSS Inc., Chicago, IL, USA). The data was presented as mean±standard deviation. The comparison for potential difference between two groups was performed with the Student’s t-test. The resulting p-values less than 0.05 were considered statistically significant.

qRT-PCR was performed to validate the results of RNA-seq analysis, 18 differentially expressed candidate genes involved in FA biosynthesis and metabolism were selected. Specific primers were designed using Primer 5.0 and were listed in Additional file 9: Table S6. Total RNA were extracted from seeds using TRIzol Reagent and treated with DNase to remove genome contaminations. The first-strand cDNA was synthesized using a RevertAid First Strand cDNA Synthesis kit (Fermentas, Vilnius, Lithuania). qRT-PCR was performed using a SYBR kit (SYBR Green I, Osaka, Japan) on a LightCycler 480 system (Roche, Basel, Switzerland). The amplification conditions were as follows: 95 °C for 1 min followed by 40 cycles of 95 °C for 10 s, and 62–68 °C for 30 s (depending on different genes). Three independent biological triplicates and two technique repeats were performed for each sample. The relative gene expression levels were normalized to inner controls β-actin and 18sRNA and calculated using 2^-ΔΔT method [42 (link)] ANOVA analysis was performed using SPSS17.0 program (SPSS Inc., Chicago, USA). All data were represented as the means ± standard error (SE, n = 3).

Data was presented as the mean ± standard deviation (n = 3 or 10). The significant difference was conducted by independent-samples T test analysis at p < 0.05 and p < 0.01 by using SPSS 17.0 program (SPSS, Inc., Chicago, IL, U.S.A.). All the experiments were carried out three times except that the experiments of enzyme activity and photosynthetic parameter were carried out ten times. All figures were drawn using the software of GraphPad prism 6 (GraphPad Software, San Diego, CA, U.S.A.) and Adobe Photoshop CS5 (Adobe, San Jose, CA, U.S.A).

Data are reported as means ± standard deviation of the mean (SD). Statistical analyses were performed using the SPSS 17.0 program (SPSS Inc., Chicago, IL). Data were analyzed using a one-way ANOVA, followed by Tukey’s honestly significant difference (HSD) post-hoc test; P-values < 0.05 were considered significant.

Spearman Rank Correlation was used to test the relation between continuous variables. We used the non-parametric Mann-Whitney U test to compare continuous and categorical variables and chi-square test for the comparison of categorical variables. Continuous variables uPA and PAI-1 were converted into binary variables to divide the patients into those with high risk and those with low risk by using limit values of 3 ng uPA / mg of proteins and 14 ng PAI-1 / mg of proteins.32 (link) Statistical analysis was performed by means of the SPSS 17.0 program. The value of p < 0.05 was considered statistically significant. uPA and PAI-1 values are shown in Table 2.