Elementsadvanced research image analysis software

Manufactured by Nikon

Nikon ElementsAdvanced Research Image-Analysis software is a comprehensive image analysis tool designed for researchers and scientists. It provides a wide range of features and capabilities for quantitative analysis of digital images, including but not limited to microscopy, histology, and material science applications.

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Lab products found in correlation

Cd11b, by BD (1 mentions) Goat anti rat antibodies, by Thermo Fisher Scientific (1 mentions) Texas red, by Thermo Fisher Scientific (1 mentions) Cd105, by BioLegend (1 mentions) Vegfr2 d5b1, by Cell Signaling Technology (1 mentions) Cd105 mj7 18, by BioLegend (1 mentions)

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Image analysis software (12 citations)

3 protocols using elementsadvanced research image analysis software

Immunohistochemical Staining of Frozen Tissue Sections

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5-μm thick frozen tissue sections were stained with CD31 (Clone
390, BD Pharmingen), CD8a (53–6.7, BD Pharmingen), CD11b (550282, BD
Biosciences), CD88(C5aR1) (sc-31240, Santa Cruz Biotechnology), C3b/iC3b/C3c,
which binds only to C3 cleavage fragments but not to intact C3 (20 (link)) (HM 1065, Hycult Biotech), C1q (HM 1096BT,
Hycult), mannan binding lectin (NB100–1502 Novus), IgM antibodies
(14-5790-81, eBioscience) and Annexin V (sc-1929, Santa Cruz Biotechnology).
Secondary antibodies included: Goat anti-rat antibodies (Invitrogen):
Streptavidin-Cy2, Texas Red, and AF 633-conjugated; and donkey anti-goat AF
488-conjugated antibodies. Stainings were quantified with Nikon Elements
Advanced Research Image-Analysis software based on analysis of at least ten
fields per section. Data is expressed as the binary area occupied by positive
cells.

Reese B., Silwal A., Daugherity E., Daugherity M., Arabi M., Daly P., Paterson Y., Woolford L., Christie A., Elias R., Brugarolas J., Wang T., Karbowniczek M, & Markiewski M.M. (2020). Complement as Prognostic Biomarker and Potential Therapeutic Target in Renal Cell Carcinoma.. Journal of immunology (Baltimore, Md. : 1950), 205(11), 3218-3229.

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Quantitative Immunofluorescence Imaging of Tissue Sections

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Frozen tissue sections 5-μm thick were stained with: CD31 (Clone 390, BD Pharmingen), CD8a (53–6.7, BD Pharmingen), NG2 (546930, R&D Systems), CD105 (MJ7/18, Biolegend), Ki-67 Ki-67 (D3B5, Cell Signaling), C3b/iC3b/C3c (HM 1065, Hycult Biotech), C1q (HM 1096BT, Hycult), mannose-binding lectin (NB100–1502 Novus), IgM (14–5790-81, eBioscience), and IgG (406705, Biolegend) antibodies. Apoptotic cells were identified by Annexin V (sc-1929, Santa Cruz). AF 488, Streptavidin-Cy2, Texas Red, and AF 633-conjugated antibodies (Invitrogen) were used as secondary reagents. Stainings in at least ten fields per section were quantified with Nikon Elements Advanced Research Image-Analysis software. Data is expressed as the area occupied by CD31/NG2-positive cells or number of cells per high-power field.

Ghouse S.M., Vadrevu S.K., Manne S., Reese B., Patel J., Patel B., Silwal A., Lodhi N., Paterson Y., Srivastava S.K., Karbowniczek M, & Markiewski M.M. (2020). Therapeutic targeting of vasculature in the premetastatic and metastatic niches reduces lung metastasis. Journal of immunology (Baltimore, Md. : 1950), 204(4), 990-1000.

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Quantifying Angiogenesis and Immune Infiltrates

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For immunofluorescence, frozen tissue sections 5-um thick were stained with anti-mouse CD31 (Clone 390; BD Pharmingen, Franklin lakes, NJ, USA), CD105 (MJ7/18; Biolegend), VEGFR2 (D5B1; Cell Signaling Technology, Danvers, MA, USA). Texas Red and AF488 conjugated antibodies were used as secondary reagents. Staining in at least 10 fields per section was quantified with Nikon Elements Advanced Research Image Analysis software. Data are expressed as the area occupied by CD31 or VEGFR2 positive cells. For safety studies, 5um thick paraffin-embedded kidney and lung sections were analyzed for tumor-infiltrating lymphocytes by H&E staining as previously described (32 (link)).

Oladejo M., Nguyen H.M., Silwal A., Reese B., Paulishak W., Markiewski M.M, & Wood L.M. (2022). Listeria-based immunotherapy directed against CD105 exerts anti-angiogenic and anti-tumor efficacy in renal cell carcinoma. Frontiers in Immunology, 13, 1038807.

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