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Dulbecco s phosphate buffered saline and tween 20

Manufactured by Thermo Fisher Scientific

Dulbecco's phosphate‐buffered saline (DPBS) is a balanced salt solution commonly used in various laboratory applications. Tween 20 is a non-ionic detergent frequently used as a surfactant in biochemical and cell culture procedures. These products are used to maintain the osmolarity and pH of solutions, as well as to facilitate cellular and molecular interactions.

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2 protocols using dulbecco s phosphate buffered saline and tween 20

1

Antioxidant Compound Extraction and Analysis

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DHM, [(2R, 3R)‐3, 5, 7‐trihydroxy‐2‐(3, 4, 5‐trihydroxyphenyl)‐2,3‐dihydrochromen‐4‐one], MW 320.25, HPLC grade, >98% (Master Herbs Inc., Pomona, CA) was used in this study. Penicillin‐streptomycin, fetal bovine serum (FBS), minimum essential medium (MEM), phosphate‐buffered saline (PBS), and Dulbecco’s phosphate‐buffered saline and Tween 20 were purchased from Thermo Fisher (Life Technologies, Foster City, CA). Dimethyl sulfoxide (DMSO) and 200 proof pure ethyl alcohol were purchased from Sigma‐Aldrich (St. Louis, MO).
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2

Chromen-4-one Compound Characterization

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DHM: [(2R, 3R)-3, 5, 7-trihydroxy-2-(3, 4, 5-trihydroxyphenyl)-2,3-dihydrochromen-4-one], MW 320.25, HPLC grade, >98% (Master Herbs Inc., Pomona, CA) was used in this study. Penicillin-streptomycin, fetal bovine serum (FBS), minimum essential medium (MEM), phosphate-buffered saline (PBS), and Dulbecco’s Phosphate-Buffered Saline and Tween 20 were purchased from ThermoFisher (Life Technologies, Foster City, CA). Dimethyl sulfoxide (DMSO) and 200 proof pure ethyl alcohol were purchased from Sigma-Aldrich (St. Louis, MO).
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