Rpmi 1640 medium powder

The anti-His₆ antibody and HRP-labeled goat anti-mouse IgG were purchased from ZSGB-Bio Co., Ltd (Beijing, China). The control antibody LN22 (Newcastle, United Kingdom) and MaxVision/HRP IHC kit were provided by Fuzhou Maixin Biotech Co., Ltd (Fuzhou, China). SP2/0 myeloma cells were stored in our laboratory. Hypoxanthine, aminopterin and thymidine supplement (HAT), Hypoxanthine and thymidine supplement (HT) were purchased from Sigma-Aldrich (Shanghai, China). RPMI 1640 medium powder and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific (Shanghai, China). Balb/c mice were from Wushi Animal Laboratory (Shanghai, China). All animal studies were approved by the Animal Ethics Committee of the Fujian Agriculture and Forestry University and have been carried out in accordance with the NIH guidelines for the care and use of Laboratory animals.

Candida albicans SC5314 and C. albicans ATCC10231, as well as four other Candida species (Table 1), were obtained from CGMCC (China General Microbiological Culture Collection Center) and kept on YPD agar at 4°C in fridge. Prior to each assay, one colony was propagated in YPD medium for 18 h at 28°C with a rotation of 140 rpm.
Fangchinoline (Purity > 98%, HPLC), DCFH‐DA‐containing ROS kit, dimethyl sulfoxide (DMSO) and N‐acetyl‐cysteine (NAC) were purchased from Solarbio (Beijing, China). MTT, XTT, fluconazole and Amphotericin B (AmB) were purchased from Sangon (Shanghai, China). PI and Calcofluor White (CFW), were purchased from Sigma (Shanghai, China). Caspofungin acetate was purchased from Aladdin Scientific (Shanghai, China). Syto 9 and RPMI‐1640 medium (powder) were purchased from Thermo (Shanghai, China). FN was dissolved in DMSO at a concentration of 10 mg/mL as stocking solution. AmB, prepared in DMSO at 1 mg/mL, was used as the positive control.