Anti gfp d5

Anti-VE-cadherin (F-8, 1 µg/ml) and DAPI were from Santa Cruz Biotechnology. Anti-E-cadherin (ab1416, 1 µg/ml), anti-Ki67 (ab15580, 0.25 µg/ml), and anti-β tubulin (ab6046, 0.25 µg/ml) were from Abcam. Anti-GM130 (clone 35, 2 µg/ml) was from BD Biosciences. Anti-VEGFA (VG-1, 2 µg/ml), anti-ZO-1 (40-2200, 1 µg/ml), rhodamine phalloidin (1 µg/ml), 70 kDa FITC-dextran and AlexaFluor 647 conjugated goat secondary antibodies were from Life Technologies. Anti-α6 integrin (MA6, 1 µg/ml) was from Millipore. Anti-HA (6E2, 0.5 µg/ml), anti-GFP (D5.1, 0.5 µg/ml), and anti-GAPDH (D16H11, 0.25 µg/ml) were from Cell Signaling Technologies. HRP-conjugated donkey anti-mouse and rabbit IgG secondary antibodies (1: 0.25 µg/ml) were purchased from Fitzgerald. Recombinant human IL-6 protein (7270-IL, 200 ng/ml), recombinant human TGFβ1 protein (240-B, 5 ng/ml), recombinant human FGF2 protein 2 (33-FB, 3 nM), anti-IL-6 (6708, 1 µg/ml), anti-IL-6Rα (MAB227, 0.5 µg/ml), and Proteome Profiler Human Cytokine Array Kit were purchased from R&D Systems. Semaxanib was purchased from Selleckchem.

The following antibodies were used in Western blot experiments: anti-CD44 (clone epr18668, Abcam, ab189524), anti-GAPDH (clone 6C5, Millipore, MAB374), anti-GFP (D5.1, Cell Signaling Technology 2956), anti-SMAD2/3 (BD Biosciences 610842), anti-Phospho-SMAD2 (45 (link)), anti-TβRI (EPR20923-13 Abcam, ab235578), anti-TβRII (EPR24349-124, Abcam, ab259360), and anti-α Tubulin (DM1A, Abcam, ab7291).