Saccharose

The putative PHB accumulation by the strain 2D1 grew in MSM culture with glucose was weighed and recorded. The amount of putative PHB extracted from the strain 2D1 pellet was calculated [27 (link)], according to the following Equation (2):
The estimations of the DCW, residual biomass, and bio-based polymer accumulation were also quantified in the presence of fructose, maltose, saccharose, sorbitol, lactose, xylose, and mannose incorporated one at a time in the MSM to replace glucose (Fisher Scientific, Goteborg, Sweden) [29 (link)].
Additional measurements of DCW, residual biomass, and PHB production were repeated by using a pretreated argan pulp, i.e., a residue obtained from the extraction of oil from the argan seeds [25 (link)].

Because of the potential for using myxobacteria as biological control agents, seven phytopathogenic prey organisms were chosen, representing a taxonomically diverse set of organisms that infect a variety of economically important plant hosts (Table 2). Gram-negative organisms were grown in LB broth (10 g/L tryptone (Fisher), 10 g/L NaCl, 5 g/L yeast extract) for 18–24 h at 37 °C, 180 rpm. Gram-positive and fungal organisms were grown in tryptone Soy Yeast Extract (17 g/L tryptone, 3 g/L soya peptone (Oxoid), 6 g/L yeast extract, 5 g/L NaCl, 2.5 g/L K₂HPO₄, 2.5 g/L glucose) or YEPS (10 g/L yeast extract, 20 g/L tryptone, 10 g/L saccharose (Fisher)), respectively, for 40 h at 30 °C, 180 rpm. Strains were maintained at 4 °C for up to two weeks on agar plates before re-plating and stored long-term as liquid glycerol stocks at −80 °C.