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2 phosphoglycerate

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2-phosphoglycerate is a chemical compound that serves as an intermediate in the glycolysis pathway, a fundamental metabolic process that converts glucose into energy. It is a naturally occurring organic phosphate ester that plays a crucial role in cellular metabolism. The primary function of 2-phosphoglycerate is to facilitate the conversion of 3-phosphoglycerate to phosphoenolpyruvate, a key step in the energy-producing glycolytic cycle.

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Lab products found in correlation

Phosphoenolpyruvate, by Merck Group (4 mentions) 3 phosphoglycerate, by Merck Group (3 mentions) Shikonin, by Merck Group (2 mentions) Mouse tnf α, by R&D Systems (2 mentions) Ifn γ 485 mi 100, by R&D Systems (2 mentions) Pyruvate, by Merck Group (2 mentions) Sypro orange, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Corning (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Malate, by Merck Group (1 mentions) Succinate, by Merck Group (1 mentions) Glutamate, by Merck Group (1 mentions) Oxaloacetate, by Merck Group (1 mentions) Malonate, by Merck Group (1 mentions) Alpha ketoglutarate, by Merck Group (1 mentions) D fructose, by Merck Group (1 mentions) Glucose 6 phosphate (g6p), by Santa Cruz Biotechnology (1 mentions) Glycerol, by Carl Roth (1 mentions)

4 protocols using 2 phosphoglycerate

1

Anti-inflammatory Protocols and Reagents

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LPS (Escherichia coli LPS 0111:B4; #L4391), 2DG (#D8375), TSA (#8552), 3-phosphoglycerate (#P8877), 2-phosphoglycerate (#19710), and phosphoenolpyruvate (#860077) were obtained from Sigma (St. Louis, MO, USA). Shikonin (#CAS 517-89-5) was obtained from EMD Millipore Corporation (Billerica, MA, USA). Mouse TNF-α (#410-MT-010) and IFN-γ (485-MI-100) were obtained from R&D Systems (Minneapolis, MN, USA). Mouse anti-HMGB1 2G7 neutralizing antibody was a generous gift from Kevin J. Tracey (The Feinstein Institute for Medical Research, Manhasset, NY, USA)32 (link).
Yang L., Xie M., Yang M., Yu Y., Zhu S., Hou W., Kang R., Lotze M., Billiar T.R., Wang H., Cao L, & Tang D. (2014). PKM2 Regulates the Warburg Effect and Promotes HMGB1 Release in Sepsis. Nature communications, 5, 4436.
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2

Thermal Stability Profiling of RsbU

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RsbU45–313 was purified as described above and buffer exchanged into PBS (Corning). DSF were performed with SyproOrange (Invitrogen) in 384-well plate (Roche) format [46 (link)]. The following potential ligands were tested: succinate, malonate, glutamate, alpha-ketoglutarate, fumarate, oxaloacetate, malate, 2-phosphoglycerate, glucose, pyruvate, phosphoenolpyruvate, and ATP (Sigma-Aldrich). All ligands were dissolved in PBS. Compounds were added to each well, followed by DSF buffer HEPES-NaOH pH7.5 (100mM), and a 10X SyproOrange dye. Reliable baselines for Tm shifts were established using 10X SyproOrange and 10 μM RsbU45–313. The mixture was heated from 20 to 85 °C. Melting curves were analyzed on Roche Tm Analysis Software.
Soules K.R., Dmitriev A., LaBrie S.D., Dimond Z.E., May B.H., Johnson D.K., Zhang Y., Battaile K.P., Lovell S, & Hefty P.S. (2019). Structural and ligand binding analyses of the periplasmic sensor domain of RsbU in Chlamydia trachomatis supports role in TCA cycle regulation. Molecular microbiology, 113(1), 68-88.
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3

Investigating Glycolytic Metabolites

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D-fructose (Product No. F0127), glyceraldehyde-3-phosphate (Product No. G5251), 3-phosphoglycerate (Product No. P8877), 2-phosphoglycerate (Product No. 79480), phosphoenolpyruvate (Product No. P7002), acetate (Product No. S2889) and pyruvate (Product No. P2256) are from Sigma-Aldrich. Glucose-6-phosphate (Product No. SC-221489A) is from Santa Cruz Biotechnology. Glycerol (Product No. 3783.2) is from Carl Roth. All chemicals were tested for their purity to exclude contamination by glucose.
Bi S., Kargeti M., Colin R., Farke N., Link H, & Sourjik V. (2023). Dynamic fluctuations in a bacterial metabolic network. Nature Communications, 14, 2173.
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4

Anti-inflammatory Protocols and Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
LPS (Escherichia coli LPS 0111:B4; #L4391), 2DG (#D8375), TSA (#8552), 3-phosphoglycerate (#P8877), 2-phosphoglycerate (#19710), and phosphoenolpyruvate (#860077) were obtained from Sigma (St. Louis, MO, USA). Shikonin (#CAS 517-89-5) was obtained from EMD Millipore Corporation (Billerica, MA, USA). Mouse TNF-α (#410-MT-010) and IFN-γ (485-MI-100) were obtained from R&D Systems (Minneapolis, MN, USA). Mouse anti-HMGB1 2G7 neutralizing antibody was a generous gift from Kevin J. Tracey (The Feinstein Institute for Medical Research, Manhasset, NY, USA)32 (link).
Yang L., Xie M., Yang M., Yu Y., Zhu S., Hou W., Kang R., Lotze M., Billiar T.R., Wang H., Cao L, & Tang D. (2014). PKM2 Regulates the Warburg Effect and Promotes HMGB1 Release in Sepsis. Nature communications, 5, 4436.
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