Tumor cell viability after IRE treatment was detected as previously described using Cell Counting Kit 8 (CCK-8; CK04, Dojindo Laboratories, Japan).51 (link) Briefly, triturated cells were seeded in 96-well flat-bottomed plates at a density of 2 × 103 in 100 μL of conditioned medium per well, and 10 μL of CCK-8 solution was added to each well. After 2 h of incubation, the absorbance of each well at a wavelength of 450 nm was quantified. To explore the death types, AO-EB (Invitrogen) double staining was performed. After 15 min of incubation, pictures were captured by confocal laser scanning microscopy (Nikon).
Ao eb
Manufactured by Thermo Fisher Scientific
The AO-EB is a laboratory instrument designed for the analysis and observation of biological samples. It provides a reliable and consistent means of performing essential tasks in the laboratory setting.
Automatically generated - may contain errors
2 protocols using ao eb
1
Measuring Tumor Cell Viability after IRE
2
Synthesis and Evaluation of Cisplatin-Metformin Hybrid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cisplatin (CPT), metformin (MET), 2-bromoisobutyryl bromide, succinic anhydride, N, N,N,N, N-pentamethyldiethylenetriamine (PMDETA), 2-Hydroxyethyl methacrylate (HEMA), triethylamine (TEA) were purchased from TCI and used as received. Sodium bore hydride (NaBH4), ferric chloride hexahydrate (FeCl3.6H2O, 99%), ferrous chloride tetrahydrate (FeCl2.4H2O, 99%), sulfuric acid (H2SO4), sodium nitrate (NaNO3), dimethyl sulfoxide (DMSO), potassium permanganate (KMnO4) and graphite were purchased from Aladdin Reagents (Shanghai, China). Thiazolyl blue tetrazolium bromide (MTT), fetal bovine serum (FBS), rhodamine B, Roswell Park Memorial Institute 1640 growth medium (RPMI 1640), AO-EB, HOECHST 33342, Penicillin-streptomycin (100X), and EDTA 0.25% (1X) were purchased from Gibco Invitrogen. The other solvents and reagents were obtained from Sinopharm Chemical Reagent and used without further purification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!