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Pe annexin 5 apoptosis detection

Manufactured by BD
Sourced in United States

PE Annexin V Apoptosis Detection is a laboratory reagent used to detect and quantify apoptosis, a type of programmed cell death. Annexin V is a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. The PE (Phycoerythrin) fluorescent label allows for the visualization and measurement of apoptotic cells using flow cytometry or fluorescence microscopy.

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4 protocols using pe annexin 5 apoptosis detection

1

Annexin V Apoptosis Assay

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After the cells were transfected, 106 cells were collected from each group. After washing once in PBS, the cells were stained with PE Annexin V Apoptosis Detection (BD Pharmingen™, New Jersey, USA) for 15 min at room temperature, and analyzed by flow cytometry (BD FACSCalibur, New Jersey, USA) to assess the proportion of apoptotic cells.
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2

Apoptosis Quantification by Flow Cytometry

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After cell transfection, 10 6 cells were collected from each group. After washing once in PBS, cells were stained with PE Annexin V and 7AAD using PE Annexin V Apoptosis Detection (BD Pharmingen™, New Jersey, USA) at room temperature for 15 minutes. We used ow cytometry (BD FACSCalibur, New Jersey, USA) to evaluate the proportion of apoptotic cells.
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3

Apoptosis Assessment by Flow Cytometry

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After the cells were transfected, 10 6 cells were collected from each group. After washing once in PBS, the cells were stained with PE Annexin V Apoptosis Detection (BD Pharmingen™, New Jersey, USA) for 15 min at room temperature, and analyzed by ow cytometry (BD FACSCalibur, New Jersey, USA) to assess the proportion of apoptotic cells.
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4

Quantifying Apoptosis via Flow Cytometry

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After cell transfection, 106 cells were collected from each group. After washing once in PBS, cells were stained with PE Annexin V and 7AAD using PE Annexin V Apoptosis Detection (BD Pharmingen™, New Jersey, USA) at room temperature for 15 minutes. We used ow cytometry (BD FACSCalibur, New Jersey, USA) to evaluate the proportion of apoptotic cells.
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