Magna riptm rna binding protein co immunoprecipitation kit

Manufactured by Merck Group

The Magna RIPTM RNA binding protein co-immunoprecipitation kit is a laboratory product manufactured by Merck Group. The kit is designed for the isolation and analysis of RNA-binding proteins and their associated RNA transcripts.

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Lab products found in correlation

Human anti ago2 antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Magna RIP RNA-Binding Protein Immunoprecipitation (RIP) Kit Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit Magna RIP RNA-Binding Protein Immunoprecipitation Kit Magna RNA immunoprecipitation (RIP) RNA-Binding Protein Immunoprecipitation Kit Magna RIP™ RNA-binding immunoprecipitation kit Magna RIP RNA-binding protein RIP Kit RIPTM RNA-binding Protein Immunoprecipitation kit RIP RNA-Binding Protein Immunoprecipitation Kit Magna RIP RNA binding protein Magna RNA-binding protein immunoprecipitation kit

2 protocols using magna riptm rna binding protein co immunoprecipitation kit

Investigating MALAT1-microRNA-216a Interaction

Cited 1 time

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Co-immunoprecipitation protocol was followed as per earlier published article by Lin et al., 2019a , Lin et al., 2019b (link). MS2-MALAT1-wt containing MS2 hairpin structure (with microRNA-216a binding site) and MS2-MALAT1-mut (without microRNA-216a binding site) were constructed. The above vector was transfected into AMSCs. Forty-eight hours later, co-immunoprecipitation was performed using Magna RIPTM RNA binding protein co-immunoprecipitation kit (Milipore). RNA purification was then carried out and qPCR quantified microRNA-216a. MS2-MALAT1-wt and MS2- MALAT1-mut were repeated three times.

Huang J., Wang F.H., Wang L., Li Y., Lu J, & Chen J. (2021). LncRNA MALAT1 promotes proliferation and migration of airway smooth muscle cells in asthma by downregulating microRNA-216a. Saudi Journal of Biological Sciences, 28(8), 4124-4131.

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Predicting circRNA-miRNA interactions

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Starbase3.0 was used to predict the sequence information of mmu_circ_0001052 and miR-106a-5p, miR-106a-5p and FGF4. Magna RIPTM RNA-binding protein co-immunoprecipitation kit (Millipore) was used for co-immunoprecipitation. Cell lysate was treated with RIP buffer containing magnetic beads conjugated with human anti-Ago2 antibody (Millipore, Billerica, MA, USA), or negative control IgG. Beads were washed with wash buffer, and the complexes were incubated with 0.1% SDS/proteinase K to remove proteins. qRT-PCR assay was carried out afterward.

Liang Z.H., Pan N.F., Lin S.S., Qiu Z.Y., Liang P., Wang J., Zhang Z, & Pan Y.C. (2022). Exosomes from mmu_circ_0001052-modified adipose-derived stem cells promote angiogenesis of DFU via miR-106a-5p and FGF4/p38MAPK pathway. Stem Cell Research & Therapy, 13, 336.

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