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Ammonium citrate tribasic

Manufactured by Merck Group
Sourced in Poland, United States

Ammonium citrate tribasic is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. The primary function of ammonium citrate tribasic is to act as a buffering agent, maintaining a specific pH in chemical reactions and solutions.

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3 protocols using ammonium citrate tribasic

1

Synthesis of Rare Earth Fluoride Compounds

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Rare earth (RE) oxides: Er2O3 and Yb2O3 (99.99%, Stanford Materials, United States) were dissolved separately in hydrochloric acid, HCl (ultra-pure, Sigma-Aldrich, 37%, Poland) in order to obtain respective rare earth chloride solutions in a concentration of 1 or 0.25 M. Ammonium fluoride, NH4F (98+%, Sigma-Aldrich, Poland) was used as the source of fluoride ions. Strontium chloride hexahydrate SrCl2·6H2O (Sigma-Aldrich, 99+%, Poland), citric acid trisodium salt dihydrate, (Sigma-Aldrich, 97%, Poland) and ammonium citrate tribasic, (Sigma-Aldrich, ≥97%, Poland), phosphate buffered saline (BioShop), poly(ethylene glycol) bis(carboxymethyl) ether (average Mn 250, Sigma-Aldrich, Poland), polyacrylic acid (average Mw 1800, Sigma – Aldrich, Poland) were used as received, without further purification. Deionized water was used for the synthesis.
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2

Molecular Cloning Protocol: Transformation and Plasmid Purification

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Bactotryptophan (code 91079-40-2); Bacto yeast (code 8013-01-2); Sodium Chloride (code 7647-14-5); Agar (code 9002-18-0); Calcium Chloride (code 10035-04-8); Isopropanol (code 67-63-0); Ethanol (code 64-17-5); SSC Buffer (code 6135-04-3); Hind III (code 81295-22-9); BGL II (code 81295-12-7); Ammonium Citrate Tribasic (code 3458-72-8); Citric Acid (code 77-92-9); Lysine (code 56-87-1); Ferrous Sulphate (code 7720-78-7) were all from Sigma Aldrich. Glycerol (code 56-81-5); Midiprep Kit, (code K0841); Agarose Gel (code 9012-36-6); Propidium Iodide (code 25535-16-4); DNA Digestion Kit (code AM1907); Ligation Buffer (code IVGN2104) were from Thermo Fischer Scientific. Ampicillin (code 69-52-3) and Tris Acetate Buffer (code 135852-26-5) were from Fischer Scientific; Blue-Orange Loading Dye was from Promega; Miniprep Kit was from Euro genomics and Ferric Chloride (code 7705-08-0) was from Merck Millipore.
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3

Protein Purification Optimized Protocol

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Ammonium citrate tribasic (Sigma-Aldrich A1332, St. Louis, MO, USA, ≥97%); PEG3350 (Hampton HR2-591, Aliso Viejo, CA, USA); Tris (VWR CHLC0497, Radnor, PA, USA, Ultra-Pure Grade); Sodium Chloride (VWR C0241, ≥99.9%); DTT (Sigma-Aldrich D0632, ≥99%); IPTG (BBI A600168, Shanghai, China, ≥99%); HEPES (Sigma-Aldrich V900477, ≥99.5%); Imidazole (Biotopped I6090, Beijing, China, ≥98%).
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