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Ammonium citrate tribasic

Manufactured by Merck Group
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Sourced in Poland, United States

Ammonium citrate tribasic is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline solid that is soluble in water. The primary function of ammonium citrate tribasic is to act as a buffering agent, maintaining a specific pH in chemical reactions and solutions.

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Lab products found in correlation

Propidium iodide, by Thermo Fisher Scientific (3 mentions) Citric acid trisodium salt dihydrate, by Merck Group (1 mentions) Poly ethylene glycol bis carboxymethyl ether, by Merck Group (1 mentions) Strontium chloride hexahydrate srcl2 6h2o, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Polyacrylic acid, by Merck Group (1 mentions) Ammonium fluoride nh4f, by Merck Group (1 mentions) Ampicillin, by Thermo Fisher Scientific (1 mentions) Ferrous sulphate, by Merck Group (1 mentions) Ligation buffer, by Thermo Fisher Scientific (1 mentions) Agarose gel, by Thermo Fisher Scientific (1 mentions) Glycerol, by Thermo Fisher Scientific (1 mentions) Tris acetate buffer, by Thermo Fisher Scientific (1 mentions) Hind 3, by Merck Group (1 mentions) Blue orange loading dye, by Promega (1 mentions) Midiprep kit, by Thermo Fisher Scientific (1 mentions) Ssc buffer, by Merck Group (1 mentions) Bacto yeast, by Merck Group (1 mentions) Calcium chloride, by Merck Group (1 mentions)

3 protocols using ammonium citrate tribasic

1

Synthesis of Rare Earth Fluoride Compounds

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Rare earth (RE) oxides: Er2O3 and Yb2O3 (99.99%, Stanford Materials, United States) were dissolved separately in hydrochloric acid, HCl (ultra-pure, Sigma-Aldrich, 37%, Poland) in order to obtain respective rare earth chloride solutions in a concentration of 1 or 0.25 M. Ammonium fluoride, NH4F (98+%, Sigma-Aldrich, Poland) was used as the source of fluoride ions. Strontium chloride hexahydrate SrCl2·6H2O (Sigma-Aldrich, 99+%, Poland), citric acid trisodium salt dihydrate, (Sigma-Aldrich, 97%, Poland) and ammonium citrate tribasic, (Sigma-Aldrich, ≥97%, Poland), phosphate buffered saline (BioShop), poly(ethylene glycol) bis(carboxymethyl) ether (average Mn 250, Sigma-Aldrich, Poland), polyacrylic acid (average Mw 1800, Sigma – Aldrich, Poland) were used as received, without further purification. Deionized water was used for the synthesis.
Przybylska D., Ekner-Grzyb A., Grześkowiak B.F, & Grzyb T. (2019). Upconverting SrF2 nanoparticles doped with Yb3+/Ho3+, Yb3+/Er3+ and Yb3+/Tm3+ ions – optimisation of synthesis method, structural, spectroscopic and cytotoxicity studies. Scientific Reports, 9, 8669.
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2

Molecular Cloning Protocol: Transformation and Plasmid Purification

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Bactotryptophan (code 91079-40-2); Bacto yeast (code 8013-01-2); Sodium Chloride (code 7647-14-5); Agar (code 9002-18-0); Calcium Chloride (code 10035-04-8); Isopropanol (code 67-63-0); Ethanol (code 64-17-5); SSC Buffer (code 6135-04-3); Hind III (code 81295-22-9); BGL II (code 81295-12-7); Ammonium Citrate Tribasic (code 3458-72-8); Citric Acid (code 77-92-9); Lysine (code 56-87-1); Ferrous Sulphate (code 7720-78-7) were all from Sigma Aldrich. Glycerol (code 56-81-5); Midiprep Kit, (code K0841); Agarose Gel (code 9012-36-6); Propidium Iodide (code 25535-16-4); DNA Digestion Kit (code AM1907); Ligation Buffer (code IVGN2104) were from Thermo Fischer Scientific. Ampicillin (code 69-52-3) and Tris Acetate Buffer (code 135852-26-5) were from Fischer Scientific; Blue-Orange Loading Dye was from Promega; Miniprep Kit was from Euro genomics and Ferric Chloride (code 7705-08-0) was from Merck Millipore.
Kundu K., Teta R., Esposito G., Stornaiuolo M, & Costantino V. (2023). A Four-Step Platform to Optimize Growth Conditions for High-Yield Production of Siderophores in Cyanobacteria. Metabolites, 13(2), 154.
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3

Protein Purification Optimized Protocol

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Ammonium citrate tribasic (Sigma-Aldrich A1332, St. Louis, MO, USA, ≥97%); PEG3350 (Hampton HR2-591, Aliso Viejo, CA, USA); Tris (VWR CHLC0497, Radnor, PA, USA, Ultra-Pure Grade); Sodium Chloride (VWR C0241, ≥99.9%); DTT (Sigma-Aldrich D0632, ≥99%); IPTG (BBI A600168, Shanghai, China, ≥99%); HEPES (Sigma-Aldrich V900477, ≥99.5%); Imidazole (Biotopped I6090, Beijing, China, ≥98%).
Qin Y., Zhou Y., Cao Y., Ren Y., Deng P., Jiang J, & Wang Z. (2023). Structural Basis of the Transcriptional Elongation Factor Paf1 Core Complex from Saccharomyces eubayanus. International Journal of Molecular Sciences, 24(10), 8730.
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