Agarose powder

Plasmid pEGFP-N1 (Clontech, Palo Alto, CA, USA) was purified using QIAGEN Plasmid Mega Kit (Qiagen GmbH, Hilden, Germany). Cysteine terminated CDX peptide was purchased from Biomatic Co. (Wilmington, USA) with a purity percentage of 99%. Bifunctional PEG (NHS-PEG-MAL, MW 2000) was purchased from Nanocs Inc. (Boston, USA). Fluorescein was purchased from Molecular Probes (Eugene, Oregon, United States). The drug Fingolimod was purchased from (AdooQ Bioscience, Centerstone Plaza, Irvine, USA). Other purchased materials were as follows: CS (Chitolytic, 17 Carlaw Ave, Toronto, ON M4M 2R7, Canada), Acetic acid (Sigma–Aldrich, St. Louis, Missouri, USA), cell culture flasks and plates (SPL, Geumgang-ro, Pocheon-si, Gyeonggi-do, Korea), tripolyphosphate (TPP) (Sigma–Aldrich, St. Louis, Missouri, USA), cellulose dialysis bag (cut off 12-14 kDa) (Sigma–Aldrich, St. Louis, Missouri, USA), agarose powder (Bio-Rad, Hercules, California, USA), SYBR^TM Green (Thermo Fisher, Waltham, Massachusetts, USA), TNBSA assay (Thermo Fisher, Waltham, Massachusetts, USA), Ellman reagent (Thermo Fisher, Waltham, Massachusetts, USA), DMEM/HG cell medium (Sigma–Aldrich, St. Louis, Missouri, USA), fetal bovine serum (Gibco^TM, Thermo Fisher, Waltham, Massachusetts, USA), Pen-Strep, and Trypsin-EDTA (Sigma–Aldrich, St. Louis, Missouri, USA).