The extraction of phytohormones and camalexin was performed as described (30 (link)). The compounds were quantified by HPLC-ESI-SRM in a Thermo Fisher TQS-Altis Triple Quadrupole Mass Spectrometer coupled to a Thermo Scientific Vanquish MD HPLC system. The chromatographic separation was carried out in a UPLC column (Agilent Eclipse Plus C18, RRHD, 1.8 um, 2.1 × 50 mm), and the compounds were eluted using water (A) and acetonitrile (B) as mobile phase at 0.6 mL/min and in a gradient elution mode as following: 10% B for 0.5 min, 10–55% of B at 4.5 min, 55–100% B at 4.7 min, 100% until 6.0 min, 100–10% B at 6.1% and 10% until 8 min. The column was kept at 55°C. The statistical significance from three replicates was evaluated by ANOVA followed by Tukey Test (Tukey HSD).
Tqs altis triple quadrupole mass spectrometer
Manufactured by Thermo Fisher Scientific
The TQS-Altis Triple Quadrupole Mass Spectrometer is a high-performance analytical instrument designed for precise and sensitive detection and quantification of chemical compounds. It utilizes triple quadrupole technology to perform tandem mass spectrometry analyses.
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2 protocols using tqs altis triple quadrupole mass spectrometer
1
Phytohormone and Camalexin Quantification
2
HPLC-ESI-SRM Quantification of Phytohormones
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The extraction of phytohormones was performed as already described (Trapp et al. 2014 (link)). The compounds were quantified by HPLC-ESI-SRM, in a Thermo Fisher TQS-Altis Triple Quadrupole Mass Spectrometer coupled to a Thermo Scientific Vanquish MD HPLC system. The chromatographic separation was carried out in a UPLC column (Agilent Eclipse Plus C18, RRHD, 1.8 μm, 2.1 × 50 mm), and the compounds were eluted using water (A) and acetonitrile (B) as mobile phase at 0.6 mL min−1 and in a gradient elution mode as following: 10% B for 0.5 min, 10% to 55% of B at 4.5 min, 55% to 100% of B at 4.7 min, 100% until 6.0 min, 100% to 10% of B at 6.1% and 10% until 8 min. The column was kept at 55 °C.
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