Enzalutamide (Cat# A10562) was purchased from Adooq Bioscience, Irvine, CA, USA, and all other reagents purchased were of GLP grade. Antibodies including Anti-AR (Cat# 5153), Anti-AR-v7 (Cat# 19672), anti-POU5F1 (OCT4) (Cat# 2750S) were purchased from Cell Signaling Technologies, Beverly, MA, USA. The anti-ALDH1 (Cat# SC-166362), anti-SOX2 (Cat# SC-365823), anti-α-GAPDH (Cat# SC-47724), goat anti-mouse IgG-HRP (Cat# SC-2005), bovine anti-goat IgG-HRP (Cat# SC-2350), and goat anti-rabbit IgG-HRP (Cat# SC-2004) antibodies were purchased from Santa Cruz Biotechnology, Dallas, TX, USA.
Enzalutamide
Manufactured by Adooq
Sourced in United States
Enzalutamide is a synthetic small-molecule androgen receptor inhibitor. It is designed to bind to and inhibit the androgen receptor, which is a protein that responds to male hormones like testosterone. The primary function of Enzalutamide is to disrupt the signaling of the androgen receptor, which plays a crucial role in the growth and development of certain types of cancer cells.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by GE Healthcare (2 mentions)
Bovine anti goat igg hrp, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions)
Anti ar v7, by Cell Signaling Technology (1 mentions)
Anti aldh1, by Santa Cruz Biotechnology (1 mentions)
Anti ar, by Cell Signaling Technology (1 mentions)
Anti sox2, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)
L glutamine, by American Type Culture Collection (1 mentions)
3 protocols using enzalutamide
1
Enzalutamide-Mediated Regulation of Stem Cell Markers
2
Enzalutamide Effects on Prostate and Brain Cells
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Androgen‐responsive human prostate cancer LNCaP and C4‐2B cells, brain neuronal BT142 cells, and glial M059K cells were used for the experiment. Prostate cancer cells were grown in RPMI 1640 (Cat#SH30027.01; GE Healthcare), and brain cells were grown in Dulbecco's Modified Eagle's Medium and Ham's F12 medium (1:1) (Cat# 30‐2006; ATCC) with an additional 0.9% glucose, 4 mM l ‐glutamine (Cat#30‐2214; American Type Culture Collection), 25 µg/ml insulin, 100 µg/ml transferrin, 20 nM progesterone, 15 µM putrescine, and 30 nM selenite. Both cell lines were supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 µg/ml streptomycin in 100 mm tissue culture plates at 37°C in a humidified atmosphere (5% CO2). All cell lines were treated with enzalutamide (20 µM) (Cat#A10562; Adooq Bioscience) for 24 h along with their respective control. After the treatment, the cells were harvested and processed for the experiment.
3
Generating Enzalutamide-Resistant Prostate Cancer Cells
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LNCaP and C4-2B cells were recurrently exposed to increasing concentrations of enzalutamide (1–20 μM) by passage in complete cell culture medium containing enzalutamide (Cat# A10562, Adooq Bioscience, Irvine, CA, USA). In each concentration of enzalutamide, the cells were grown in RPMI 1640 (GE Healthcare, Chicago, IL, USA) for a week, allowing them to acclimatize and propagate for a minimum of six months. The resistant cells generated from 20 µM enzalutamide were maintained in media containing 5 µM enzalutamide, referred to as LNCaP and C4-2B enzalutamide-resistant cells. The parental cell lines were propagated in dimethyl sulfoxide as the vehicle for the same time period. The final concentration of the vehicle did not exceed 0.1% in all treatments. Both cell lines were maintained in similar culture conditions.
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