Anti cd39 pe

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-CD39-PE is a fluorescently-labeled antibody that binds to the CD39 cell surface protein. It is used for the identification and analysis of cells expressing CD39 in flow cytometry applications.

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2 protocols using anti cd39 pe

Comprehensive Immunophenotyping by Flow Cytometry

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For flow cytometry analysis cells were washed with PBS containing 0.5% BSA, either surface stained with anti-CD4-eFluor-780, CD4-FITC (eBioscience), anti-CD25-PE, anti-CTLA4-PE, anti-CD39-PE, anti-Helios-PE, anti-TIGIT-PE or fixed, permeabilized, and stained with anti-, Anti-Ki67-PE, anti-Foxp3-APC and isotype controls antibodies (eBioscience) (1:100) in dark. Samples were analyzed using CyAn ADP Analyzer (Beckman and Coulter) and data analysis was done using Summit v4.3 software.

Kumar P., Alharshawi K., Bhattacharya P., Marinelarena A., Haddad C., Sun Z., Chiba S., Epstein A.L, & Prabhakar B.S. (2017). Soluble OX40L and JAG1 Induce Selective Proliferation of Functional Regulatory T-Cells Independent of canonical TCR signaling. Scientific Reports, 7, 39751.

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Comprehensive Immune Cell Profiling

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Fluorochrome-coupled antibodies specific for human antigens: anti-CD4-FITC, anti-CD127-PE-Cy7, anti-CD62L-PE, anti-CCR4-PE, anti-CCR7-PE, anti-CCR6-PE, anti-CCR10-PE, anti-CXCR3-PE-Cy5, anti-HLA-DR-FITC, anti-CD25-PE-Cy7, anti-CD39-PE, anti-CD44-PE-Cy7, anti-CD45RA-PE-Cy5, anti-GITR-PE, anti-CD45RO-PE, and anti-CD278-PE (all from eBioscience, USA) were used for cell surface staining. Monoclonal antibodies (mAbs)anti-Foxp3-PE, anti-CTLA-4-PE-Cy7, anti-Helios-FITC, and anti-IDO (indolamine-2,3-dioxygenase)-PE-Cy7 (all from BD Pharmingen, USA) were used for intracellular staining. Quantitative analysis was performed using FlowJo software.

He X., Li S., Zhang J., Cao L., Yang C., Rong P., Yi S., Ghimire K., Ma X, & Wang W. (2021). Benefit of Belatacept in Cord Blood-Derived Regulatory T Cell-Mediated Suppression of Alloimmune Response. Cell Transplantation, 30, 09636897211046556.

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