Rnalater ice solution
RNAlater-ICE solution is a laboratory reagent designed to stabilize and preserve RNA in biological samples. It is intended for use in the collection, storage, and transportation of RNA samples prior to RNA extraction and analysis.
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5 protocols using rnalater ice solution
Total RNA Extraction and cDNA Synthesis
Whole Lung RNA Extraction and Microarray
Lung tissues were homogenized in a Tissue Lyser (Qiagen, UK) 2x 2 min at 30 rpm and centrifuged at 13.2K rpm for 3 min to remove cell debris. Total RNA was extracted using RNeasy mini columns (Qiagen, UK) with an additional step of genomic DNA removal through agDNA eliminator column.
RNA was quantified using a Nanodrop One C spectrophotometer (Labtech International) and quality checked using an RNA Screen Tape on aTape Station instrument (Agilent Technologies). All the extracted RNAs used in the subsequent analysis had an RNA integrity number (RIN e )>6. Total RNA (200ng) was labelled with Cy3-CTP using the Low input Quick Amp One Color labelling kit (Agilent Technologies) and hybridized onto whole genome 8 X 60K mouse microarrays v2 (AMADID 074809) following the manufacturer's instructions. The microarrays were washed and scanned using an Agilent microarray scanner G2505C.
Dual RNA and Protein Extraction
Quantitative Analysis of AGER Expression in Human Lung Tissue
RNA Extraction from HCC Cells and Tissues
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