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Tween 20

Manufactured by Maclin Biochemical Technology
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Sourced in China

Tween 20 is a non-ionic surfactant. It is a polyoxyethylene (20) sorbitan monolaurate. Tween 20 is commonly used as a detergent and emulsifier in various laboratory applications, including protein purification, Western blotting, and immunoassays.

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Lab products found in correlation

Sodium caseinate, by Merck Group (1 mentions) Resveratrol, by Maclin Biochemical Technology (1 mentions) Polydatin, by Merck Group (1 mentions) Chloroauric acid, by Merck Group (1 mentions) Horseradish peroxidase hrp, by Maclin Biochemical Technology (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Chloroplatinic acid, by Merck Group (1 mentions) Ethylenediaminetetraacetic acid, by Maclin Biochemical Technology (1 mentions) Ethanolamine hydrochloride, by Merck Group (1 mentions) Nicl2, by Maclin Biochemical Technology (1 mentions) Sodium hydroxide (naoh), by Maclin Biochemical Technology (1 mentions) Simplicity 185, by Merck Group (1 mentions) Kh2po4, by Maclin Biochemical Technology (1 mentions) K2hpo4, by Maclin Biochemical Technology (1 mentions) Sodium chloride (nacl), by Maclin Biochemical Technology (1 mentions) N hydroxysuccinimide, by Merck Group (1 mentions) N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions) Peroxidase conjugated affinipure goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Chromatographic grade acetonitrile, by Merck Group (1 mentions)

4 protocols using tween 20

1

Bioactive Compound Enriched Emulsions

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Sodium caseinate, polydatin (HPLC grade, >95%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). WPI was purchased from Davisco International, Inc. (Russel, MN, USA). Tween 20 (Biotechnology grade, ≥99%), resveratrol (trans-isomer, ≥98%), fish oil and peppermint oil were respectively obtained from Macklin Co. (Shanghai, China), Sango Biotech Co. (Shanghai, China), Chaopu Co. (Shanghai, China), Ltd. and Zixin Biotechnology Co., Ltd. (Shanghai, China). Sunflower oil (Duoli brand) was purchased from a local market (Wuxi, China). MCT (C8:C10 = 60:40) was purchased from Yong sheng Industry and Trade Co., Ltd. (Guangzhou, China). Other materials of analytical grade were purchased from Sino-Pharm CNCM Ltd. (Shanghai, China).
Chen Y., Cheng H, & Liang L. (2023). Effect of Oil Type on Spatial Partition of Resveratrol in the Aqueous Phase, the Protein Interface and the Oil Phase of O/W Emulsions Stabilized by Whey Protein and Caseinate. Antioxidants, 12(3), 589.
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2

SARS-CoV-2 NP Antigen Detection ELISA

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Chloroauric acid (HAuCl4⋅3H2O), chloroplatinic acid (H2PtCl6⋅6H2O), trisodium citrate, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MI, United States). Tween-20, horseradish peroxidase (HRP), hydrogen peroxide (30% H2O2), and tetramethylbenzidine (TMB) were purchased from Macklin (Shanghai, China). The magnetic beads (MBs), with a diameter of 5 μm, were purchased from Beaverbio (Suzhou, China). The reference standard of SARS-CoV-2 NP antigen was purchased from Bioeast Biotech (Hangzhou, China). The NP (SARS-CoV-2) Detection ELISA Kit was purchased from Biodragon Immunotechnologies (Beijing, China).
Liu B., Wu Z., Liang C., Lu J., Li J., Zhang L., Li T., Zhao W., Fu Y., Hou S., Tang X, & Li C. (2021). Development of a Smartphone-Based Nanozyme-Linked Immunosorbent Assay for Quantitative Detection of SARS-CoV-2 Nucleocapsid Phosphoprotein in Blood. Frontiers in Microbiology, 12, 692831.
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3

SARS-CoV-2 Spike Protein Binding Assay

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), and ethanolamine hydrochloride were acquired from Sigma (St. Louis, MO, USA). K2HPO4, KH2PO4, NaCl, NaOH, NiCl2, ethylenediaminetetraacetic acid (EDTA), Tween-20 and heparin were obtained from Macklin Biochemical Technology (Shanghai, China). His-tagged SARS-CoV-2 S1 protein and its polyclonal antibody (anti-SARS-CoV-2, 95% purity, type rabbit) were purchased from Sangon Biotech (Shanghai, China). CM-dextran sensor chips were obtained from Biosensing Instrument Inc. (Tempe, AZ, USA). Tris-NTA sensor was prepared as described previously [49 (link)]. Other reagents were of analytical purity and used as received. Deionized water was purified by a Millipore system (Simplicity 185, Millipore Corp, Billerica, MA).
Jiang M., Dong T., Han C., Liu L., Zhang T., Kang Q., Wang P, & Zhou F. (2022). Regenerable and high-throughput surface plasmon resonance assay for rapid screening of anti-SARS-CoV-2 antibody in serum samples. Analytica Chimica Acta, 1208, 339830.
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4

Imidacloprid ELISA Assay Protocol

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Imidacloprid monoclonal antibody and ovalbumin-coated hapten (OVA-Hapten) were provided by Zhejiang University (Hangzhou, China). Peroxidase-conjugated AffiniPure goat antimouse IgG was purchased from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA). Imidacloprid standard (100 mg/kg) was procured from Beijing Manhage Bio-Technology Co., Ltd. (Beijing, China). Acetonitrile (chromatographic grade) was procured from Merck AG (Darmstadt, Germany). TMB (3,3′,5,5′-Tetramethylbenzidine) single-component substrate solution, phosphate buffer saline tablets, and tris (hydroxymethyl) aminomethane were secured from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). HRP-chemiluminescent reagents A and B were supplied by Beijing Keyuebio Technology Co., Ltd. (Beijing, China). Albumin (bovine serum) was obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Tween 20 was acquired from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). A 96-well opaque assay plate was obtained from Shanghai Jing An Biological Technology Co., Ltd. (Shanghai, China). An immuno clear flat-bottom 96-well plate (item #439454#) was supplied by Thermo Fisher Scientific (Pittsburgh, PA, USA).
Zhai R., Chen G., Liu G., Huang X., Xu X., Li L., Zhang Y., Xu D, & Abd El-Aty A.M. (2023). Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables. Foods, 12(1), 196.
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