The largest database of trusted experimental protocols

Hispur nickel nitrilotriacetic acid agarose ni nta resin

Manufactured by Thermo Fisher Scientific

HisPur™ Nickel-nitrilotriacetic acid-agarose (Ni-NTA) resin is a chromatography resin designed for the purification of His-tagged recombinant proteins. The resin consists of nickel-charged nitrilotriacetic acid (Ni-NTA) coupled to an agarose matrix.

Automatically generated - may contain errors

2 protocols using hispur nickel nitrilotriacetic acid agarose ni nta resin

1

Protein Purification and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oligonucleotide primers were synthesized by Integrated DNA Technologies or Sigma-Aldrich. Recombinant plasmid DNA was purified with a QIAprep® Spin Miniprep Kit from Qiagen. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification Kit from GE Healthcare. DNA sequencing was performed by Beckman Coulter Genomics and Eton Bioscience. Optical densities of E. coli cultures were determined with a DU 730 Life Sciences UV/Vis spectrophotometer (Beckman Coulter) by measuring absorbance at 600 nm. All chemicals and solvents were obtained from Sigma-Aldrich except where noted.
During protein purification, proteolysis was inhibited by adding Pierce™ Protease Inhibitor tablets (EDTA free; Thermo Scientific) to lysis buffer. Affinity chromatography and SDS-PAGE analysis were conducted using HisPur™ Nickel-nitrilotriacetic acid-agarose (Ni-NTA) resin (Thermo) and 10% or 4–15% Mini-PROTEAN TGX Precast SDS-PAGE gels (Bio-Rad) with 2× Laemmli sample buffer (Bio-Rad). Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo).
+ Open protocol
+ Expand
2

Protein Purification and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Oligonucleotide primers were synthesized by Integrated DNA Technologies or Sigma-Aldrich. Recombinant plasmid DNA was purified with a QIAprep® Spin Miniprep Kit from Qiagen. Gel extraction of DNA fragments and restriction endonuclease clean up were performed using an Illustra GFX PCR DNA and Gel Band Purification Kit from GE Healthcare. DNA sequencing was performed by Beckman Coulter Genomics and Eton Bioscience. Optical densities of E. coli cultures were determined with a DU 730 Life Sciences UV/Vis spectrophotometer (Beckman Coulter) by measuring absorbance at 600 nm. All chemicals and solvents were obtained from Sigma-Aldrich except where noted.
During protein purification, proteolysis was inhibited by adding Pierce™ Protease Inhibitor tablets (EDTA free; Thermo Scientific) to lysis buffer. Affinity chromatography and SDS-PAGE analysis were conducted using HisPur™ Nickel-nitrilotriacetic acid-agarose (Ni-NTA) resin (Thermo) and 10% or 4–15% Mini-PROTEAN TGX Precast SDS-PAGE gels (Bio-Rad) with 2× Laemmli sample buffer (Bio-Rad). Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!